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It is generally accepted that the functional compartmentalization of eukaryotic cells is reflected by the differential occurrence of proteins in their compartments. The location and physiological function of a protein ar...

In this work we briefly describe the most relevant features of WSXM, a freeware scanning probe microscopy software based on MS-Windows. The article is structured in three different sections: The introduction is a perspec...

We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in ...

Molecular excitation by the simultaneous absorption of two photons provides intrinsic three-dimensional resolution in laser scanning fluorescence microscopy. The excitation of fluorophores having single-photon absorption...

Subjects / keywords: Electron microscope; Cryo-electron microscopy; Microscopy; Anisotropy; Materials science; Motion (physics); Optics; Electron; Biophysics; Physics; Nanotechnology; Nuclear magnetic resonance

Subjects / keywords: Fluorophore; Microscopy; Resolution (logic); Photoactivated localization microscopy; Fluorescence-lifetime imaging microscopy; Optics; Fluorescence microscope; Diffraction; Fluorescence; Materials science; Image resoluti...

Subjects / keywords: microscopy, materials science, instrumentation, electron microscopy, biological science; Science: Natural history (General): Microscopy

Subjects / keywords: optical microscopy, fluorescence microscopy, super-resolution microscopy, (low- and high voltage) electron microscopy, correlative microscopy, scanning probe microscopy; Science: Natural history (General): Microscopy

Current knowledge of the nervous system, particularly the brain, is the result of technological advances and the collective effort of many scientists. However, it was Santiago Ramón y Cajal (1852–1934) who played a cr...

We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to approximately 2 ...

The scanning tunneling microscope is proposed as a method to measure forces as small as ${10}^{\ensuremath{-}18}$ N. As one application for this concept, we introduce a new type of microscope capable of investigating sur...