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Morphometry of bovine blastocysts produced in vitro in culture media with antioxidants cysteamine or oily extract of Lippia origanoides

Article

Morphometry of bovine blastocysts produced in vitro in culture media with antioxidants cysteamine or oily extract of Lippia origanoides

N.V. Sollecito et al · Universidade Federal de Minas Gerais, Escola de Veterinária · 2021 · ISSN 1678-4162

ABSTRACT This study aimed to evaluate the ultrastructural morphometry of bovine embryos produced in vitro grown at different concentrations of antioxidants. After in vitro maturation and fertilization, the presumptive zy...

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RNA-Seq study of in vivo-produced single male and female bovine blastocysts

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RNA-Seq study of in vivo-produced single male and female bovine blastocysts

Baoshan Cui et al · Sociedade Brasileira de Zootecnia · 2023 · ISSN 1806-9290

ABSTRACT The objective of this study was to identify differentially expressed genes (DEG) between females and males in early bovine embryos. Superovulated cattle (n = 4) in the age span of 16–18 months were artificiall...

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Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

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Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

Maria Helena Coelho Cruz et al · Sociedade Brasileira de Zootecnia · 2014 · ISSN 1806-9290

This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five differ...

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Stepwise vitrification of in vitro produced buffalo Blastocysts

Article

Stepwise vitrification of in vitro produced buffalo Blastocysts

Abd-Allah,S.M · Universidad de Córdoba · 2011

The purpose of this study was to evaluate the use of a stepwise vitrification as a method for cryopreservation of in vitro-produced (IVP) buffalo blastocysts and to compare the results with post-thaw survival rate of buf...

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Embryonic Stem Cell Lines Derived from Human Blastocysts

Article

Embryonic Stem Cell Lines Derived from Human Blastocysts

James A. Thomson; Joseph Itskovitz‐Eldor; Sander S. Shapiro; Michelle Waknitz; Jennifer J. Swiergiel; Vivienne S. Marshall; Jeffrey M. Jones · Science · 1998

Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but ...

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Allergenic response induced by Pterobothrium crassicolle (Cestoda: Trypanorhyncha) extracts in murine model

Article

Allergenic response induced by Pterobothrium crassicolle (Cestoda: Trypanorhyncha) extracts in murine model

Danuza Pinheiro Bastos Garcia de Mattos et al · Colégio Brasileiro de Parasitologia Veterinária · 2024 · ISSN 1984-2961

Abstract The aim of this study was to determine the allergenic activity of components present in crude extracts of Pterobothrium crassicolle plerocerci (CPE) and blastocysts (CBE) obtained from Micropogonias furnieri in ...

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Association of recombinant bovine somatotropin (rBST) with equine chorionic gonadotropin (eCG) on antral follicle count and oocyte production in Holstein and Tabapuã heifers

Article

Association of recombinant bovine somatotropin (rBST) with equine chorionic gonadotropin (eCG) on antral follicle count and oocyte production in Holstein and Tabapuã heifers

Hévea de Morais et al · Sociedade Brasileira de Zootecnia · 2012 · ISSN 1516-3598

The objective of this study was to investigate whether the use of rbST and eCG prior to ultrasound-guided follicular aspiration (OPU) improves oocyte yield and quality in Tabapuã and Holstein heifers. The study was cond...

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Avaliação do Desenvolvimento de Embriões Bovinos Pós-Biópsia: um Modelo para Treinamento Evaluation of the Post-Biopsy Development of Bovine Embryos: Proposal of a Training Model

Article

Avaliação do Desenvolvimento de Embriões Bovinos Pós-Biópsia: um Modelo para Treinamento Evaluation of the Post-Biopsy Development of Bovine Embryos: Proposal of a Training Model

Carlos Gilberto Almodin et al · Federação Brasileira das Sociedades de Ginecologia e Obstetrícia · 1999 · ISSN 0100-7203

Objetivo: montar um protocolo animal para estudo e treinamento em biópsia embrionária. Métodos: ovários de vaca obtidos em abatedouro eram transportados ao laboratório onde os oócitos aspirados eram maturados. Post...

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Clinical correlation of apoptosis in human luteinized granulosa cells

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Clinical correlation of apoptosis in human luteinized granulosa cells

Shengrong Du et al · BMC · 2026 · ISSN 1757-2215

Abstract Background Apoptosis is a continuous process throughout follicular development and plays a critical role in normal ovarian physiology. Although the key apoptotic molecule-encoding genes have been well characteri...

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DNA fragmentation, transgene expression and embryo development after intracytoplasmic injection of DNA-liposome complexes in IVF bovine zygotes

Article

DNA fragmentation, transgene expression and embryo development after intracytoplasmic injection of DNA-liposome complexes in IVF bovine zygotes

Vichera, G. et al · Cambridge University Press · 2012 · ISSN 0967-1994

Summary This study was designed to evaluate the quality and viability of bovine embryos produced by in vitro fertilization (IVF), after intracytoplasmic injection of pCX-EGFP-liposome complexes or pBCKIP2.8-liposome comp...

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Efeito de diferentes meios de cultivo no desenvolvimento e proporção do sexo de embriões bovinos produzidos in vitro Effect of different culture media on development and sex ratio of bovine embryos fertilized in vitro

Article

S.G.T. Gilardi et al · Universidade Federal de Minas Gerais, Escola de Veterinária · 2004 · ISSN 0102-0935

Avaliou-se o efeito da suplementação de meios de cultivo sobre o desenvolvimento e proporção do sexo de embriões bovinos fertilizados in vitro. Complexos cumulus-oócitos obtidos de ovários de matadouro foram matur...

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Efeito do número da passagem e do gênero das células doadoras de núcleo no desenvolvimento de bovinos produzidos por transferência nuclear Effect of culture time and gender of nuclei donor cells on bovine development produced by nuclear transfer

Article

Giovana Krempel Fonseca Merighe et al · Sociedade Brasileira de Zootecnia · 2010 · ISSN 1516-3598

Objetivou-se com este trabalho avaliar o efeito do número da passagem e do sexo das células doadoras de núcleo no desenvolvimento embrionário e fetal após transferência nuclear. Para isso, oócitos bovinos foram ma...

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Efeito do transporte no desenvolvimento de embriões bovinos cultivados in vitro a fresco ou reaquecidos após vitrificação Effect of transportation on development of fresh or vitrified-warmed bovine embryos

Article

Alessandra de Almeida Ramos et al · Sociedade Brasileira de Zootecnia · 2006 · ISSN 1516-3598

Avaliou-se a viabilidade de embriões bovinos cultivados in vitro, a fresco ou reaquecidos após vitrificação, depois de transportados por 6 ou 12 horas. Oócitos obtidos de folículos de ovários coletados em matadour...

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Effect of early addition of bone morphogenetic protein 5 (BMP5) to embryo culture medium on in vitro development and expression of developmentally important genes in bovine preimplantation embryos

Article

Effect of early addition of bone morphogenetic protein 5 (BMP5) to embryo culture medium on in vitro development and expression of developmentally important genes in bovine preimplantation embryos

Garcia, Elina Vanesa et al · Elsevier Science Inc · 2015 · ISSN 0093-691X

Previous studies have reported that bone morphogenetic protein 5 (BMP5) is differentially expressed in the isthmus of bovine oviducts and it is present in the oviductal fluid. However, the specific action of this factor ...

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Effect of the Air Filtration System Replacement on Embryo Quality in the Assisted Reproduction Laboratory

Article

Effect of the Air Filtration System Replacement on Embryo Quality in the Assisted Reproduction Laboratory

Karine Queiroz Poletto et al · Federação Brasileira das Sociedades de Ginecologia e Obstetrícia · 2018 · ISSN 0100-7203

Abstract Improving infrastructural conditions of the in vitro fertilization laboratory, such as the air quality, has profound positive effects on embryo culture. Poor environmental conditions reduce the rate of embryo fo...

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Effects of Hydrosalpinx on Endometrial Implantation Failures: Evaluating Salpingectomy in Women Undergoing in vitro fertilization

Article

Effects of Hydrosalpinx on Endometrial Implantation Failures: Evaluating Salpingectomy in Women Undergoing in vitro fertilization

Antonio Palagiano et al · Federação Brasileira das Sociedades de Ginecologia e Obstetrícia · 2021 · ISSN 0100-7203

Abstract Hydrosalpinx is a disease characterized by the obstruction of the salpinx, with progressive accumulation in the shape of a fluid-filled sac at the distal part of the tuba uterina, and closed to the ovary. Women ...

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Embryotrophic effect of a short-term embryo coculture with bovine luteal cells

Article

Embryotrophic effect of a short-term embryo coculture with bovine luteal cells

Maruri, Alejandro et al · Elsevier Science Inc · 2018 · ISSN 0093-691X

The coculture with somatic cells is an alternative to improve suboptimal in vitro culture (IVC) conditions and promote embryo development. Several cell types have been used for this purpose, but there is no information a...

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First Baby Born in Brazil after Simultaneous Diagnosis through Non-Invasive and Conventional PGT-A

Article

First Baby Born in Brazil after Simultaneous Diagnosis through Non-Invasive and Conventional PGT-A

Marcos Iuri Roos Kulmann et al · Federação Brasileira das Sociedades de Ginecologia e Obstetrícia · 2022 · ISSN 0100-7203

Abstract Non-invasive preimplantation genetic testing for aneuploidies (niPGT-A) aiming to assess cell-free embryonic DNA in spent culturemedia is promising, especially because it might overcome the diminished rates of i...

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Genetic analysis of biopsy-related droplets in patients undergoing PGT-A and its potential application

Article

Genetic analysis of biopsy-related droplets in patients undergoing PGT-A and its potential application

Zhiqing Huang et al · Frontiers Media S.A · 2026 · ISSN 1664-2392

PurposePreimplantation genetic testing for aneuploidy (PGT-A) is essential for selecting embryos free from chromosomal abnormalities before transfer. However, challenges such as detection failures, mosaicism, and the lim...

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Gobioides broussonnetii (Gobiidae): a new host forPterobothrium crassicolle (Trypanorhyncha) on Marajó Island, northern Brazil

Article

Gobioides broussonnetii (Gobiidae): a new host forPterobothrium crassicolle (Trypanorhyncha) on Marajó Island, northern Brazil

Marcela Videira et al · Colégio Brasileiro de Parasitologia Veterinária · ISSN 1984-2961

In the present study, the cestoid Pterobothrium crassicolle Diesing, 1850 (Trypanorhyncha), was observed parasitizing specimens of the violet goby (Gobioides broussonnetii Lacepède, 1800) collected from the estuary of t...

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Larval cestodes infecting commercial fish of Alexandria coast along the Mediterranean Sea: morphology and phylogeny

Article

Larval cestodes infecting commercial fish of Alexandria coast along the Mediterranean Sea: morphology and phylogeny

Kareem Morsy et al · Colégio Brasileiro de Parasitologia Veterinária · 2022 · ISSN 1984-2961

Abstract Members of the order Trypanorhyncha are cestode parasites that are frequently found infecting the muscles of several marine fish species, affecting fish health and resulting in consumers’ rejection. Seventy–...

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Nova técnica para contagem do número de células de blastocistos

Article

Nova técnica para contagem do número de células de blastocistos

E.P. Costa et al · Universidade Federal de Minas Gerais, Escola de Veterinária · 2010 · ISSN 1678-4162

A simplified, fast, and innovative method was developed to count the total cell number in blastocysts. Murine blastocysts (N = 195) were used in this study. They were obtained after 10h culture of initial blastocysts, co...

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Phospholipid composition and resistance to vitrification of in vivo blastocyst of a Brazilian naturalized porcine breed

Article

Phospholipid composition and resistance to vitrification of in vivo blastocyst of a Brazilian naturalized porcine breed

J.F.W. Sprícigo et al · Universidade Federal de Minas Gerais, Escola de Veterinária · ISSN 1678-4162

ABSTRACT Piau porcine blastocysts were submitted to MALDI-TOF to identify the main phospholipids (PL). After that, in vivo blastocysts (D6) were vitrified (n=52), non-vitrified were used as control (n=42). After warming,...

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Relative expression of mRNAs related to cavitation process in bovine embryos produced in vivo and in vitro Expressão relativa de mRNAs relacionados com o processo de cavitação em embriões bovinos produzidos in vivo e in vitro

Article

Sabine Wohlres-Viana et al · Sociedade Brasileira de Zootecnia · 2011 · ISSN 1516-3598

The objectives of this work were to identify and to evaluate possible differences on gene expression of aquaporins and Na/K-ATPases transcripts between embryos in vivo and in vitro produced. For each group, 15 blastocyst...

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