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Subjects / keywords: nuclear dna, dna methylation, dna replication, dna testing, dna-based hybrid materials, dna-based sensors; Science: Chemistry: Organic chemistry: Biochemistry

This work describes a new method for extracting genomic DNA from heavy oil-contaminated soils. This method combines soil washes using three washing solutions with enzymatic lysis (SW-EL method). The suitability of the SW...

The chemically synthesizable quantum emitters such as quantum dots (QDs), fluorescent nanodiamonds (FNDs), and organic fluorescent dyes can be integrated with an easy-to-craft quantum nanophotonic device, which would be ...

The genome size was surveyed in 13 Notolathyrus species endemic to South America by flow cytometry and analyzed in an evolutionary and biogeographic context. A DNA content variation of 1.7-fold was registered, and fo...

Endocrine disruptors, such as estrogen, are chemical substances with the potential to alter the hormonal balance of organisms. Their origin can be natural or artificial, and they can act at very low doses. The estrogen 1...

Flow cytometric analysis of DNA content was performed on 28 samples of canine mammary tumors. Nine of them were benign and 19 were malignant. All benign tumors and 11 malignant tumors (57.9%) were diploid (P

Summary This study was designed to evaluate the quality and viability of bovine embryos produced by in vitro fertilization (IVF), after intracytoplasmic injection of pCX-EGFP-liposome complexes or pBCKIP2.8-liposome comp...

Objective: Sleep deprivation and poor quality of sleep are a current issue in our society. In animal models, sleep deprivation can induce diverse alterations, including oxidative stress in several organs. A highly active...

DNA barcoding is a technique in which species identification is performed by using DNA sequences from a small fragment of the genome, with the aim of contributing to a wide range of ecological and conservation s...

Abstract Background Epigenetic mechanisms including DNA methylation are assumed to play crucial roles in the maintenance of genome integrity, regulation of gene expression and development, and their increasing exploitati...

BACKGROUND: It is not yet known whether DNA methylation levels can be used to accurately predict age across a broad spectrum of human tissues and cell types, nor whether the resulting age prediction is a biologically mea...

Molecular genetic maps are commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs) among the progeny of a sexual cross. Here we describe a new DNA polymorphism assay based o...

A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2',3'-dideoxy and ar...

Subjects / keywords: Ciencia; Ciencias de la Vida; Biología

Subjects / keywords: Ciencia; General

Subjects / keywords: Ciencia; General

The development of a novel microfluidic device that includes a circular microchannel and integrated electrodes for DNA electrophoresis is reported. The geometry of the separation channel and the arrangement of the embedd...

ABSTRACT Many tropical trees have high canopies and their leaves are not accessible. Thus, the use of tissue from a more accessible organ (cambium) for DNA extraction may be an alternative for molecular studies. We adapt...

Abstract Purpose Patients with nasopharyngeal carcinoma (NPC) exhibiting persistent EBV DNA positivity throughout treatment (a “D-D-D” trajectory) represent a high-risk group with undefined management strategies. Thi...

The objective of this study was to evaluate the effects of air-drying preservation on llama sperm DNA. Semen collections were carried out using electroejaculation under general anesthesia. A total of 16 ejaculates were p...

This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes (A, B, C, D, an...

Abstract Background Cervical cancer remains the fourth most common malignancy among women worldwide, with limited therapeutic success in advanced and recurrent stages. Conventional treatments, including radiotherapy and ...

BackgroundThe methylation status of cell-free DNA (cfDNA) shows promise for the clinical detection of cervical cancer and its precursors; however, its measurement in liquid biopsies is hindered by low cfDNA yields and te...

BackgroundAlterations in ribosomal DNA copy number (rDNA-CN) and global DNA methylation have been associated with genomic instability and various diseases. However, their relevance in myocardial infarction (MI) has not b...