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Subjects / keywords: CRISPR; Genome engineering; Cas9; Genome editing; Guide RNA; Biology; Computational biology; Nuclease; Genome; Homology directed repair; Synthetic biology; Non-homologous end joining

Advanced materials, serving as the core material foundation underpinning economic transformation, national defense security, and technological revolution, directly determine national competitiveness through their researc...

ABSTRACT Genome engineering plays a crucial role in the rapidly growing fields of metabolic engineering and synthetic biology. Chromosomal integration and stable expression of functional genes or large metabolic pathways...

Bacteria and archaea have evolved adaptive immune defenses, termed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems, that use short RNA to direct degradation of foreign n...

The advent of facile genome engineering using the bacterial RNA-guided CRISPR-Cas9 system in animals and plants is transforming biology. We review the history of CRISPR (clustered regularly interspaced palindromic repeat...

Functional elucidation of causal genetic variants and elements requires precise genome editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune ...

Subjects / keywords: CRISPR; Cas9; Biology; Genome engineering; Genome editing; Computational biology; Genome; Genetics; Gene

Subjects / keywords: high-throughput computation, high-throughput preparation and characterization, high-throughput experiments, materials database, materials big-data technologies, digitalized and intelligent materials r&d; Technology: Elec...

Human induced pluripotent stem cells (iPSCs) are transforming adoptive cell therapy by combining unlimited self-renewal, broad differentiation potential, and high amenability to genome engineering. These attributes make ...

Abstract Psammochloa villosa is a perennial herbaceous plant belonging to tribe Stipeae (Poaceae). It is mainly distributed in the arid and sandy Inner Mongolia Plateau and adjacent areas, demonstrating remarkable resili...

Food texture is increasingly recognized as a determinant of nutritional safety, metabolic regulation, and overall health. However, conventional approaches to texture modification rely primarily on hydrocolloids, mechanic...

Abstract Background Several cyanobacterial strains are known to contain a high number of genome copies and elevated cell cycle dynamics. We systematically investigated the ploidy of the model cyanobacterium Synechocystis...

ABSTRACT The validity of genetic engineering of virus genes as an essential tool for the development of more effective and safe vaccines has been proven recently with SARS CoV-2 mRNA and vectored vaccines. The epidemiolo...

ABSTRACT Upstream open reading frames (uORFs) are regulatory elements present in the 5′ leaders of mRNA that can significantly impact downstream gene expression in eukaryotes. In crop engineering, editing of uORFs can ...

Desde os primórdios o homem buscou selecionar as plantas alimentícias para maior produtividade. O conhecimento da estrutura do DNA permitiu que a engenharia genética se desenvolvesse consideravelmente fornecendo ferra...

Subjects / keywords: animal biotechnology, microbiology, genome engineering, domesticated animals, synthetic biology, immunogenetics; Technology: Chemical technology: Biotechnology

Subjects / keywords: applied biochemistry, computational chemistry and drug discovery, genome engineering, cell engineering, antibody engineering, enzyme engineering; Science: Chemistry: Organic chemistry: Biochemistry; Science: Biology (Gen...

Subjects / keywords: sequencing technologies, genome editing and genome engineering, imaging technologies, single-molecule methods, computational methods, machine learning; Technology: Chemical technology: Biotechnology; Science: Chemistry: ...

Terapia gênica é o tratamento baseado na introdução de genes sadios com uso de técnicas de DNA recombinante. O primeiro teste clínico bem-sucedido dessa técnica foi divulgado em 1990. Em que pese a ocorrência, em...

Subjects / keywords: Redundancy (engineering); Gene; Set (abstract data type); Database; Computer science; Computational biology; Ensembl; Biology; Data mining; Genomics; Genetics; Genome

Subjects / keywords: biosystems design, genome editing, synthetic genomics, biodesign theory, biological engineering, biodesign applications; Technology: Chemical technology: Biotechnology; Science: Biology (General): Genetics

Subjects / keywords: synthetic biology, metabolic engineering, natural products, enzyme engineering, biosystems engineering, genome editing; Technology: Chemical technology: Biotechnology; Science: Biology (General)

Abstract The plastic pollution crisis urges innovative recycling solutions. Promising approaches especially for polyester-containing wastes include enzymatic hydrolysis and microbial upcycling. For efficient enzymatic hy...