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Sequencing technologies — the next generation
Text / resource
Text / resource
Sequencing technologies — the next generation
Michael L. Metzker · Nature Reviews Genetics · 2009
Subjects / keywords: DNA sequencing; Biology; Computational biology; Emerging technologies; Data science; Selection (genetic algorithm); Computer science; Genetics; DNA; Artificial intelligence
Idioma English
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The Evolution of Gene Sequencing Technologies: Unveiling Genetic Architecture of Nonsyndromic Orofacial Clefts
Article
Article
The Evolution of Gene Sequencing Technologies: Unveiling Genetic Architecture of Nonsyndromic Orofacial Clefts
Haolang Zhao et al · Wiley · 2026 · ISSN 1469-5073
Nonsyndromic orofacial clefts (NSOC) are common congenital craniofacial developmental defects. Current evidence suggests that genetic factors, environmental exposures, and their interactions jointly contribute to the dev...
LCC LCC:GeneticsIdioma English
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Guía para la integración de las TIC en el aula de idiomas
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E-book
Guía para la integración de las TIC en el aula de idiomas
Dominguez Miguela, Antonia; Fernández Santiago, Miriam · Universidad de Huelva · 2006 · ISBN 9788417288006
La presente guía pretende ser un instrumento sencillo de conocimiento general de los medios, herramientas y posibilidades que las nuevas tecnologías de la información y la comunicación (TICs) ofrecen a los y las doce...
Idioma Spanish
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Epidemiological investigation and analysis of an outbreak of foodborne disease in school caused by Staphylococcus aureus enterotoxin
Article
Article
Epidemiological investigation and analysis of an outbreak of foodborne disease in school caused by Staphylococcus aureus enterotoxin
JIANG Wenjun et al · The Editorial Office of Chinese Journal of Food Hygiene · 2025 · ISSN 1004-8456
ObjectiveTo investigate and analyze the epidemiological characteristics of an outbreak of foodborne disease in school K， explore key procedures and response measures for on-site management of such outbreaks， and to p...
LCC TENDOkZvb2QgcHJvY2Vzc2luZyBhbmQgbWFudWZhY3R1cmU~; LCC:Nutrition. Foods and food supplyIdioma zho
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Fabrications, Applications and Challenges of Solid-state Nanopores: A Mini Review
Article
Article
Fabrications, Applications and Challenges of Solid-state Nanopores: A Mini Review
Zifan Tang et al · SAGE Publishing · 2016 · ISSN 1847-9804
Nanopore sensors are expected to be one of the most promising next generation sequencing technologies, with label-free, amplification-free and high-throughput fea‐ tures, as well as rapid detections and low cost. Solid...
LCC LCC:Materials of engineering and construction. Mechanics of materials; TENDOlRlY2hub2xvZ3k~Idioma English
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A ribose world: Current status and future challenges of plant RNA biology
Article
Article
A ribose world: Current status and future challenges of plant RNA biology
Marquardt, Sebastian et al · Oxford University Press · 2023 · ISSN 2203-2207
Elucidation of the cellular functions of RNAs represents one of the most exciting research areas in modern life sciences. RNAs play crucial roles in plant biology, including developmental regulation and environmental res...
Idioma English
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Alternative splicing in human cells exposed to ionizing radiation: a comprehensive review of ex vivo and in vivo studies
Article
Article
Alternative splicing in human cells exposed to ionizing radiation: a comprehensive review of ex vivo and in vivo studies
Leberle, Jeronimo Hector et al · Taylor & Francis Ltd · 2025 · ISSN 0955-3002
Purpose: This study reviews how ionizing radiation (IR) induces alternative splicing (AS) in non-tumor and tumor cells under both ex vivo and in vivo irradiation conditions. The relevance and limitations of IR-induced AS...
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Chromosome-level genome assembly reveals the genetic mechanisms underlying elite waterlogging tolerance in Actinidia rufa
Article
Article
Chromosome-level genome assembly reveals the genetic mechanisms underlying elite waterlogging tolerance in Actinidia rufa
Qiong Zhang et al · Springer · 2026 · ISSN 2948-1104
Abstract Actinidia rufa is a valuable species for hybridization and introgressive breeding of new kiwifruit cultivars due to its exceptional tolerance to waterlogging. In this study, a chromosome-level genome of A. rufa ...
LCC TENDOlBsYW50IGN1bHR1cmU~Idioma English
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Comparison of state-of-the-art error-correction coding for sequence-based DNA data storage
Article
Article
Comparison of state-of-the-art error-correction coding for sequence-based DNA data storage
Andreas L. Gimpel et al · Nature Portfolio · 2026 · ISSN 2041-1723
Abstract Many codecs with different error-correction approaches have been implemented for DNA data storage to date. However, no studies have systematically benchmarked codec implementations to establish their current sta...
LCC TENDOlNjaWVuY2U~Idioma English
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Genetic Prediction of Sepsis Severity Based on the Detection of MCP-1 Gene Polymorphism in Patients with Acquired Immunological Disorders
Article
Article
Genetic Prediction of Sepsis Severity Based on the Detection of MCP-1 Gene Polymorphism in Patients with Acquired Immunological Disorders
N. Matselski et al · Rockefeller University Press · 2026 · ISSN 3065-8993
Background and AimsAcquired immunodeficiency in patients with hematological oncology is a serious and common complication, significantly impacting prognosis and quality of life. High doses of cytostatic drugs and the mal...
LCC LCC:Immunologic diseases. AllergyIdioma English
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Guiding the global evolution of cytogenetic testing for hematologic malignancies
Article
Article
Guiding the global evolution of cytogenetic testing for hematologic malignancies
Akkari, Yassmine M.N. et al · American Society of Hematology · 2022 · ISSN 2273-2284
Cytogenetics has long represented a critical component in the clinical evaluation of hematologic malignancies. Chromosome banding studies provide a simultaneous snapshot of genome-wide copy number and structural variatio...
Idioma English
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Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattle
Article
Article
Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattle
Goszczynski, Daniel Estanislao et al · Wiley Blackwell Publishing, Inc · 2014 · ISSN 0001-2815
Polymorphisms of the BoLA-DRB3 gene are located primarily in the second exon [antigen binding site (ABS)] and, to a lesser extent, in the upstream regulatory region (URR). It can be hypothesised that exon 2 and the URR a...
Idioma English
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Invited review: Novel methods and perspectives for modulating the rumen microbiome through selective breeding as a means to improve complex traits: Implications for methane emissions in cattle
Article
Article
Invited review: Novel methods and perspectives for modulating the rumen microbiome through selective breeding as a means to improve complex traits: Implications for methane emissions in cattle
González Recio, Oscar et al · Universidad de Costa Rica · 2023 · ISSN 1871-1413
The rumen microbiome is responsible for methane emission in ruminants. The study of microbes in the rumen has attracted great interest in the last decade. High-throughput sequencing technologies have been key in expandin...
Idioma English
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Recent developments in nucleic acid based techniques for use in rumen manipulation
Article
Article
Recent developments in nucleic acid based techniques for use in rumen manipulation
Christopher McSweeney et al · Sociedade Brasileira de Zootecnia · 2009 · ISSN 1516-3598
Nucleic acid-based techniques which can be used to characterise complex microbial communities without incubation are now being employed regularly in ruminant nutrition studies. Conventional culture-based methods for enum...
LCC LCC:Animal cultureIdioma English
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Reconsidering rumen microbial consortia to enhance feed efficiency and reduce environmental impact of ruminant livestock production systems Consórcios microbianos no rúmen para melhorar a eficiência alimentar e reduzir o impacto ambiental dos sistemas de produção animal
Article
Article
Reconsidering rumen microbial consortia to enhance feed efficiency and reduce environmental impact of ruminant livestock production systems Consórcios microbianos no rúmen para melhorar a eficiência alimentar e reduzir o impacto ambiental dos sistemas de produção animal
Jeffrey Lynn Firkins · Sociedade Brasileira de Zootecnia · 2010 · ISSN 1516-3598
Because cultivation-based approaches to assess rumen microbiology assess only the minority of microbial groups and can miss opportunities to assess important functions, molecular-based approaches have flourished in micro...
LCC LCC:Animal cultureIdioma English
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mist: a hierarchical Bayesian framework for detecting differential DNA methylation dynamics in single-cell data
Article
Article
mist: a hierarchical Bayesian framework for detecting differential DNA methylation dynamics in single-cell data
Daoyu Duan et al · Nature Portfolio · 2026 · ISSN 2041-1723
Abstract Recent advancements in single-cell DNA methylation (scDNAm) sequencing technologies have enabled the profiling of epigenetic landscapes at unprecedented resolution, offering insights into cellular heterogeneity,...
LCC TENDOlNjaWVuY2U~Idioma English
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Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform
Article
Article
Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform
James J. Kozich; Sarah L. Westcott; Nielson T. Baxter; Sarah K. Highlander; Patrick D. Schloss · Applied and Environmental Microbiology · 2013
Rapid advances in sequencing technology have changed the experimental landscape of microbial ecology. In the last 10 years, the field has moved from sequencing hundreds of 16S rRNA gene fragments per study using clone li...
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Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads
Article
Article
Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads
Ryan R. Wick; Louise M. Judd; Claire L. Gorrie; Kathryn E. Holt · PLoS Computational Biology · 2017
The Illumina DNA sequencing platform generates accurate but short reads, which can be used to produce accurate but fragmented genome assemblies. Pacific Biosciences and Oxford Nanopore Technologies DNA sequencing platfor...
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CD-HIT: accelerated for clustering the next-generation sequencing data
Article
Article
CD-HIT: accelerated for clustering the next-generation sequencing data
LiMin Fu; Beifang Niu; Zhengwei Zhu; Sitao Wu; Weizhong Li · Bioinformatics · 2012
SUMMARY: CD-HIT is a widely used program for clustering biological sequences to reduce sequence redundancy and improve the performance of other sequence analyses. In response to the rapid increase in the amount of sequen...
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DnaSP 6: DNA Sequence Polymorphism Analysis of Large Data Sets
Article
Article
DnaSP 6: DNA Sequence Polymorphism Analysis of Large Data Sets
Julio Rozas; Albert Ferrer-Mata; Juan Carlos Sánchez-DelBarrio; Sara Guirao‐Rico; Pablo Librado; Sebastián E. Ramos‐Onsins; Alejandro Sánchez‐Gracia · Molecular Biology and Evolution · 2017
We present version 6 of the DNA Sequence Polymorphism (DnaSP) software, a new version of the popular tool for performing exhaustive population genetic analyses on multiple sequence alignments. This major upgrade incorpor...
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Fast and accurate short read alignment with Burrows–Wheeler transform
Article
Article
Fast and accurate short read alignment with Burrows–Wheeler transform
Heng Li; Richard Durbin · Bioinformatics · 2009
MOTIVATION: The enormous amount of short reads generated by the new DNA sequencing technologies call for the development of fast and accurate read alignment programs. A first generation of hash table-based methods has be...
Idioma English
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Pilon: An Integrated Tool for Comprehensive Microbial Variant Detection and Genome Assembly Improvement
Article
Article
Pilon: An Integrated Tool for Comprehensive Microbial Variant Detection and Genome Assembly Improvement
Bruce J. Walker; Thomas Abeel; Terrance Shea; Margaret Priest; Amr Abouelliel; Sharadha Sakthikumar; Christina A. Cuomo; Qiandong Zeng · PLoS ONE · 2014
Advances in modern sequencing technologies allow us to generate sufficient data to analyze hundreds of bacterial genomes from a single machine in a single day. This potential for sequencing massive numbers of genomes cal...
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featureCounts: an efficient general purpose program for assigning sequence reads to genomic features
Article
Article
featureCounts: an efficient general purpose program for assigning sequence reads to genomic features
Yang Liao; Gordon K. Smyth; Wei Shi · Bioinformatics · 2013
MOTIVATION: Next-generation sequencing technologies generate millions of short sequence reads, which are usually aligned to a reference genome. In many applications, the key information required for downstream analysis i...
Idioma English
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An Integrated Genomic Analysis of Human Glioblastoma Multiforme
Article
Article
An Integrated Genomic Analysis of Human Glioblastoma Multiforme
D. Williams Parsons; Siân Jones; Xiaosong Zhang; Jimmy Lin; Rebecca Leary; Philipp Angenendt; Parminder K. Mankoo; Hannah Carter · Science · 2008
Glioblastoma multiforme (GBM) is the most common and lethal type of brain cancer. To identify the genetic alterations in GBMs, we sequenced 20,661 protein coding genes, determined the presence of amplifications and delet...
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