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Imaging Intracellular Fluorescent Proteins at Nanometer Resolution

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Imaging Intracellular Fluorescent Proteins at Nanometer Resolution

Eric Betzig; George H. Patterson; Rachid Sougrat; O. Wolf Lindwasser; Scott G. Olenych; Juan S. Bonifacino; Michael W. Davidson; Jennifer Lippincott‐Schwartz · Science · 2006

We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to approximately 2 ...

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Ultralow power demand in fluorescence nanoscopy with digitally enhanced stimulated emission depletion

Article

Ultralow power demand in fluorescence nanoscopy with digitally enhanced stimulated emission depletion

Wang Luwei et al · Wiley · 2020 · ISSN 2192-8614

Stimulated emission depletion (STED) microscopy breaks the optical diffraction barrier and has become a powerful tool for biological study. However, its application for in vivo study is limited because of its high demand...

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