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Xenopus laevis oocytes were injected with [14C] phe-tRNA and the fate of the aminoacyl moiety was studied. The radioactive phenylalanine is gradually hydrolized off the tRNA once inside the cell. The rate of deacylation ...

Materias / palabras clave: Chemistry; Coomassie Brilliant Blue; Chromatography; Bradford protein assay; Sodium dodecyl sulfate; Sodium; Absorption (acoustics); Colorimetry; Microgram; Biochemistry; Organic chemistry; In vitro

Materias / palabras clave: Lowry protein assay; Chemistry; Chromatography; Protein precipitation; Denaturation (fissile materials); Bradford protein assay; Biochemistry; High-performance liquid chromatography; Nuclear chemistry; Casein

Materias / palabras clave: Endonuclease; DNA; Restriction enzyme; Agarose; Oligonucleotide; Chemistry; Molecular biology; DNA polymerase; DNA clamp; Polymerase; Polymerase chain reaction; Biochemistry

Materias / palabras clave: Fumigation; Soil water; Biomass (ecology); Incubation; Chemistry; Environmental chemistry; Agronomy; Ecology; Biology; Biochemistry

Materias / palabras clave: Nitrate; Nitrite; Chemistry; Chromatography; Detection limit; Derivatization; Urine; Distilled water; Dilution; Mass spectrometry; Biochemistry; Organic chemistry

Materias / palabras clave: Thiobarbituric acid; Chemistry; Lipid peroxide; Peroxide; Chromatography; Carbon tetrachloride; Peroxide value; Malondialdehyde; Absorbance; Lipid oxidation; Hydrogen peroxide; Biochemistry

BACKGROUND/AIMS: Human parvovirus B19 (B19V) may cause inflammatory cardiomyopathy (iCMP) which is accompanied by endothelial dysfunction. The B19V capsid protein VP1 contains a lysophosphatidylcholine producing phosphol...

Materias / palabras clave: Reactive oxygen species; Reactive nitrogen species; Cell biology; Oxidative stress; DNA damage; Nitric oxide; Biochemistry; Mitochondrion; Mitochondrial ROS; Chemistry; Intracellular; Biology

Intact ribonucleic acid (RNA) has been prepared from tissues rich in ribonuclease such as the rat pancreas by efficient homogenization in a 4 M solution of the potent protein denaturant guanidinium thiocyanate plus 0.1 M...

Materias / palabras clave: Chemistry; Autoxidation; Chloroplast; Lipid peroxidation; Photochemistry; Malondialdehyde; Oxygen; Chlorophyll; Chlorophyll a; Light intensity; Biochemistry; Nuclear chemistry

Materias / palabras clave: Reactive oxygen species; Glutathione reductase; Biochemistry; Antioxidant; Chemistry; Superoxide dismutase; Oxidative stress; Glutathione; Ascorbic acid; Glutathione peroxidase; Catalase; Superoxide

Autophagy is an important intracellular degradative process that delivers cytoplasmic proteins to lysosome for degradation. Dysfunction of autophagy is implicated in several human diseases, such as neurodegenerative dise...

Materias / palabras clave: RNA extraction; Chloroform; Extraction (chemistry); Phenol; Chemistry; RNA; Thiocyanate; Chromatography; Isolation (microbiology); Phenol extraction; Yield (engineering); Biochemistry

Materias / palabras clave: RNA extraction; Chloroform; Phenol; Extraction (chemistry); Chemistry; Thiocyanate; Chromatography; RNA; Isolation (microbiology); Phenol extraction; Yield (engineering); Biochemistry

Materias / palabras clave: Superoxide dismutase; Polyacrylamide; Acrylamide; Chemistry; Polyacrylamide gel electrophoresis; Dismutase; Biochemistry; Enzyme; Staining; Molecular biology; Chromatography; Biology

Materias / palabras clave: Disulfide bond; Chemistry; Heme; Biochemistry; Enzyme

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectrophoretic analysis of the major polypeptides of the human erythrocyte membraneG. Fairbanks, Theodore L. Steck, and D. F. H. WallachCite this: Biochemistry 1971, 10, 13, 2...

High-affinity nucleic acid ligands for a protein were isolated by a procedure that depends on alternate cycles of ligand selection from pools of variant sequences and amplification of the bound species. Multiple rounds e...