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A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus, greatly simplifies the procedure and, by enabling the amplifi...

We describe a new molecular approach to analyzing the genetic diversity of complex microbial populations. This technique is based on the separation of polymerase chain reaction-amplified fragments of genes coding for 16S...

High-affinity nucleic acid ligands for a protein were isolated by a procedure that depends on alternate cycles of ligand selection from pools of variant sequences and amplification of the bound species. Multiple rounds e...

Materias / palabras clave: Scaling; Polymer physics; Physics; Statistical physics; Polymer; Theoretical physics; Mathematics; Geometry; Nuclear magnetic resonance

We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been...

Materias / palabras clave: Nick translation; DNA; Molecular biology; DNA polymerase; DNA polymerase I; Polymerase; Biology; Endonuclease; Restriction enzyme; Nucleotide; DNA clamp; Escherichia coli

The purpose of this study was to attain and characterize ternary complexes of simvastatin, β-cyclodextrin (βCD) and different polymers, and then select those that lead to a greater increase in drug solubility. The comp...

Polymeric and lipid nanocapsules suspensions of the natural compound curcumin were prepared in order to overcome limitations associated with its clinical applications, such as poor aqueous solubility and susceptibility t...

In the present investigation, extended release tablets of metoprolol succinate were developed using cellulose derivatives and natural gums as matrix formers and were evaluated for its extended release characteristics. Th...

"En esta tesis, se estudia el uso de iniciadores multifuncionales para la polimerización en masa de estireno. El objetivo general es desarrollar modelos matemáticos detallados, y a la vez genéricos, que permitan descr...

"Chemical Enhanced Oil Recovery by alkali, surfactant and/or polymer flooding could be o good option to produce remaining oil from brown fields. One parameter that affects performance and economics of a CEOR process is h...

Materias / palabras clave: polymer blending, material composite, polymer rheology and molding, biodegradable and renewable polymer, polymer characterization and testing, polymer processing techniques and equipmentpolymer blending; Technology: Chem...

Materias / palabras clave: plastics, polymers, elastomers, polymer manufacturing, polymer product design, environmental impact; Technology: Chemical technology: Polymers and polymer manufacture

In coastal areas, microplastics (MPs) can deposit in sediment, allowing it to be ingested by benthic organisms, like mussels, thus creating a possible transfer to humans. The aim of this study is to evaluate MPs pollutio...

Abstract Objective Streptococcus agalactiae is an important pathogen in neonates and pregnant women. Neonatal invasive infections due to S. agalactiae are life-threatening and preventive strategies for this challenge o...

This study proposes a deep-hole directional blasting technology based on polymer energy charge packages for roof cutting and pressure relief as a solution to difficult roadway support and poor stability of coal pillars d...

We developed a method to graft a tripeptide (glutathione) onto 5-hydroxy-1,4-naphthoquinone, an electropolymerizable molecule. The resulting thin conducting polymer presents a well-defined and stable electroactivity ...

The behaviours of several cationic polyelectrolytes (chitosan; polyquaternium-4; diethylaminoethyl dextran; polyquaternium-10, and aminoacrylmethacrylate copolymer, Eudragit® E100) at a polarised liquid/liquid interface...

Background: Molecular sieves are materials with selective sorption properties, and are used to separate or load mixtures of compounds based on their molecular shape and size. These materials are usually made from porous ...

Abstract Polymer molecular weight plays a central role in determining the balance between electrostatic and steric stabilization in polymer-coated colloidal systems, yet its influence on nanoparticle accessibility at sof...

Influence of weight polymer variation towards sodium fluoride patch physical properties. Fluoride has been proven as a material for strengthen teeth. Many fluoride modalities are available nowadays, and the recent is sod...

Steady-state and time-resolved emission spectroscopy (TRES) of the medium-sensitive probes 4-aminophthalimide (4-AP) and 6-propionyl-2-(dimethylamino)naphthalene (Prodan) were performed at 77 and 298 K in vacuum-sealed t...

Non-toxic biodegradable linear poly(ester-urethane-urea)s with different hydrophilic character were synthesized from poly(ε-caprolactone) as macrodiol, l-lysine diisocyanate (LDI) and ethyl ester l-lysine or l-ornithine...

Abstract Carbon dioxide enhanced oil recovery (CO2-EOR) offers dual benefits of increasing hydrocarbon production and sequestering anthropogenic CO2; however, conventional CO2 flooding suffers from unfavorable mobility r...