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Abstract To investigate antibiotic resistance genes (ARGs), conjugative plasmids, and virulence genes in Swedish waterborne Escherichia spp., water samples were collected from urban freshwater and Baltic Sea beaches and ...

A genetic vaccine consisting of the bovine herpesvirus-1.2a (BHV-1.2a) glycoprotein D (gD) gene under the control of the cytomegalovirus immediate-early promoter/enhancer was generated and administered to sheep intramusc...

Materias / palabras clave: Plasmid; Transformation (genetics); Escherichia coli; Plasmid preparation; Biology; Incubation; Dithiothreitol; T-DNA Binary system; DNA; Transformation efficiency; Chemistry; Genetics

Abstract The study included thirty-six isolates of rhizobia bacteria were isolated from the nodules located on the roots of nine types of leguminous family plants that were planted in four areas of the city of Mosul for ...

A 5-year-old female presented at age 2 with recurrent respiratory and ear infections, aphthous ulcers, and severe neutropenia (absolute neutrophil count [ANC] = 400 cells/mcl). Immunological testing at age 4 showed neutr...

We recently identified Galectin-1 (Gal-1), a β-galactoside-binding lectin, as a novel immune regulator in neuroblastoma (NB). Here, we characterized the tolerogenic function of Gal-1 within the CD8+ T cell compartment a...

Abstract Despite the significance of antibiotics in treating bacterial infections, antibiotic resistance is continuously increasing, thus posing a significant threat. In addition to strains resistant to individual drugs,...

ObjectiveTo evaluate the antimicrobial resistance characteristics and molecular epidemiological features of Escherichia coli in retail whole chickens in Beijing， including resistance rates， resistance profiles， ser...

ABSTRACT Mycobacterium smegmatis is nonpathogenic and fast-growing and is usually used as a model species of Mycobacterium. Studying basic metabolic mechanisms is crucial for accelerating mycobacterial research. Although...

Abstract Therapeutic human papillomavirus (HPV) DNA vaccine is an attractive option to control existed HPV infection and related lesions. The two early viral oncoproteins, E6 and E7, are continuously expressed in most HP...

Abstract Background Carbapenem-resistant Citrobacter freundii is increasingly reported and recognized as an opportunistic host of mobile carbapenemase-encoding elements in healthcare-associated settings. However, lineage...

Abstract Background The emergence of tmexCD-toprJ, a plasmid-mediated resistance-nodulation-division efflux pump gene cluster conferring resistance to tigecycline, poses a critical public health threat. While its prevale...

The objective of the present study was to report the molecular cloning and determination of the sequences N-methyl-D-aspartate subunit-1 receptor (NMDAR-1) in the hypothalamus of sheep. The re-amplified DNA template for ...

Abstract We present the complete genome sequence of Sphingomonas sp. gentR, a strain exhibiting high-level resistance to gentamicin (MIC = 40 mg/mL). The genome was assembled from hybrid Illumina and Nanopore seque...

Summary This study was designed to evaluate the quality and viability of bovine embryos produced by in vitro fertilization (IVF), after intracytoplasmic injection of pCX-EGFP-liposome complexes or pBCKIP2.8-liposome comp...

Objectives: Isolation of colistin- and carbapenem-resistant Klebsiella pneumoniae (CCR-Kp) is increasing in hospital settings worldwide, which is related to increased morbidity, mortality and healthcare costs. The aim of...

This study succeeded in developing hairy roots from the hypocotyledonous stems of chamomile Matricaria chamomilla L. plant seedlings by Ri plasmid isolated from Agrobacterium rhizogenes ATCC 15834 using direct injection ...

The World Health Organization has identified ST23 carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) as a critical public health threat. Through China’s national surveillance system (BRICS), we identifi...

Abstract Antimicrobial resistance and emergence of MDR and XDR Salmonella enterica subsp. enterica serovar Typhi is a major threat in Pakistan. Failure of treatment with conventional antibiotics leads to importance of ex...

Mobile genetic elements (MGEs) such as bacteriophages and plasmids profoundly shape microbial community structure and drive horizontal gene transfer across ecosystems. Wastewater treatment systems, with their high cell d...

Abstract Carbapenemase-producing Acinetobacter baumannii (CP-Ab) is a critical priority pathogen. Between October 2019 and September 2020, a multicentre study was conducted at four Ethiopian hospitals: Tikur Anbessa and ...

ABSTRACT Transcriptional regulation of metabolic operons is important for optimal carbohydrate use and for mitigating the accumulation of toxic intermediates. Here, we characterize SL1344_3500, encoding a putative IclR-l...

Abstract Objective Tissue-resident memory T (TRM) cells play a key role as “immune sentinels” in mucosal immune protection. However, the molecular regulatory mechanisms underlying the formation and long-term resid...

Abstract Background Vascular calcification is a major pathological process driving cardiovascular morbidity and mortality, yet effective therapies are lacking. This study aims to identify novel causal plasma proteins and...