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Food texture is increasingly recognized as a determinant of nutritional safety, metabolic regulation, and overall health. However, conventional approaches to texture modification rely primarily on hydrocolloids, mechanic...

Abstract Leptospirosis is a worldwide zoonosis caused by pathogenic bacteria of the genus Leptospira. Lipopolysaccharide (LPS) is an immunodominant and protective antigen for Leptospira, but its biosynthesis remains poor...

Materias / palabras clave: Genética médica; Enfermedades hereditarias; Sistema nervioso; Enfermedades; CRISPR (Genética)

Materias / palabras clave: Ingeniería genética; Evolución humana; ADN; Biotecnología

Yellow fever (YF) is an epidemic disease caused by the yellow fever virus (YFV). Historically, it has caused several epidemics and continues to result in fatalities in South Sudan and other regions today. Due to its limi...

ntroducción: Últimas investigaciones en la biología celular y molecular del oído interno han aportado evidencias que apoyan nuevas modalidades terapéuticas en medicina regenerativa para restablecer la audición. Rec...

Abstract Precise identification of low-frequency EGFR L858R mutations is crucial for targeted therapy and prognosis in non-small cell lung cancer (NSCLC). Here, we present CIRCLE-F/V (CRISPR-based Integrated Restriction-...

Duck adenovirus 3 (DAdV-3) was firstly identified in Muscovy ducklings exhibiting slight pericardial effusion, swelling, and hemorrhaging in the kidneys, which is a poorly characterized duck virus. This study established...

Abstract Brucellosis is a common zoonotic disease caused by Brucella and remains a globally concerning public health issue. Timely and effective detection methods are crucial for clinical diagnosis. We developed a novel ...

Abstract Genome-wide CRISPR-Cas9 knockout screens are often used to experimentally evaluate gene function. However, the efficacy of individual sgRNAs targeting unique genes varies and is difficult to integrate. In this s...

Abstract The CRISPR‐Cas system constitutes an adaptive immune mechanism in prokaryotes that defends against mobile genetic elements. Within the perpetual co‐evolutionary arms race between bacteria and their viral pre...

Polyphenol Oxidases (PPOs) catalyze the conversion of phenolic substrates to quinones, leading to the formation of dark-colored precipitates in fruits and vegetables. This process, known as enzymatic browning, is the cau...

Abstract The CRISPR–Cas9 system provides adaptive immunity against invading genetic elements through a dual-RNA-guided DNA cleavage mechanism. This system relies on the precise assembly of a ribonucleoprotein (RNP) com...

Abstract Congenital cytomegalovirus (CMV) infection is the leading non-genetic cause of infant hearing loss worldwide, and a significant cause of neurodevelopmental disabilities. Reliance on polymerase chain reaction (PC...

Functional elucidation of causal genetic variants and elements requires precise genome editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune ...

Materias / palabras clave: CRISPR; Genome engineering; Cas9; Genome editing; Guide RNA; Biology; Computational biology; Nuclease; Genome; Homology directed repair; Synthetic biology; Non-homologous end joining

ABSTRACT Introduction Antimicrobial resistance (AMR) is a global health crisis that poses a devastating and specific threat to immunocompromised cancer patients, who are heavily reliant on antibiotics to navigate high‐...

Abstract Endocytosis and intracellular trafficking of plasma membrane (PM)-resident proteins are crucial for regulating their activity and polarized localization. However, the molecular mechanisms underlying these proces...

Abstract Sickle Cell Disease (SCD) remains a significant global health burden, disproportionately affecting low- and middle-income countries (LMICs), particularly in sub-Saharan Africa. Recent advancements in gene-based ...

Abstract Background Bacillus thuringiensis is widely employed for biological control. It can effectively suppress populations of various mosquito species, including Aedes aegypti. However, the precise mechanism underlyin...