Anti-aging Properties from Gold Nanoparticles Serum using Fig Leaf Extract (Ficus carica L.)

Indonesian women saw premature aging as a serious problem; 60% of female respondents felt less confident due to the symptoms of premature aging they experienced. Fig leaves (Ficus carica L.) are a natural ingredient containing flavonoid compounds that have the potential to act as antioxidants and bio-reduction in the biosynthesis of gold nanoparticles that have anti-aging properties. This research aims to determine the antiaging activity of fig leaf serum in inhibiting collagenase enzymes and fibroblast cell proliferation. The research design used a true experimental method by macerating fig leaves. Formulation of 90 μL fig leaf extract gold nanoparticles was carried out in serum preparations varying in the concentration of 5, 10, and 15%. The characteristics of the serum preparation were tested for organoleptic, homogeneity, pH, and viscosity. Antioxidant activity was compared using the DPPH method with Vitamin C. The test for inhibiting skin-degrading enzymes is carried out by inhibiting the collagenase enzyme. Test the cell viability of fig leaf gold nanoparticles against HDFa cells using the MTT assay method. The 15% serum results obtained strong antioxidant activity with an IC50 of 21.63 μg/ml, showed good collagenase enzyme inhibition of 88.1%, and could increase cell viability after exposure to H2O2 by 93.22%. It can be concluded that gold nanoparticle serum from fig leaf had the potential for antiaging activity and the stability of the new "fig leaf extract-gold nanoparticle serum" formulation for further improvement as a new antiaging cosmetic.