Ultrafiltration Fibers like Bioreactors

A polysulfone ultrafiltration membrane with pectinase physically immobilized on it by a dynamic formation method was used to examine the potential of these reactive membranes in applications involving solutions containing pectin. The effect of various operational parameters such as: pH of enzyme and pectin solutions, NaCl, retentate flow rate, and enzyme (Ce) and substrate (Cp) concentrations on the production of reducing compounds expressed as galacturonic acid (Ca), was investigated. It was found that the maximum Ca values were obtained when: (i) enzyme solution to immobilize, without NaCl, had pH values between 4.2 and 4.6 and enzyme concentration from 5.0 to 7.5 mg/mL; (ii) pectin solution had initial pH values between 4.2 and 5.0 and a concentration of 3 mg/mL; and (iii) retentate flow rate was 0.025 L/min Fil: Carrin, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina Fil: Ceci, Liliana Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina