STUDY GENETIC DISTANCE OF OLIVE CULTIVARS USING MICROSATELLITE MARKER

The genetic distance between ten cultivated olive was determined through Microsatellite, method at DNA level from Baghdad, and Al-Mosel, position. The genetic distance matrix, UPGMA, and NJ dendrograms, and PCA were performed. The Assessment of genetic relation among olive varieties cultivated in Iraq was ensured exactly through these markers. The study showed Fifteen, Simple, sequence, repeat loci were studied, and produced, 243 amplified, fragment 243 of these loci (99.16%) were polymorphic. Five primers generated sufficient polymorphic bands to figure out the genetic diversity among the cultivars., the results showed that cultivar Shami is a hybrid of two cultivars Arbqween and Nepali while the two cultivars Sorani and Manzenllo with 100% similarity. Which indicates due to belong to the same origin. Establishing the genetic relationship of those varieties would be suitable to use for future genetic improvement studies in olive cultivars. Keywords: Microsatellite, PCR, pedigree, polymorphic, Olive