Polysaccharides from the green seaweed Codium decorticatum: Structure and cell wall distribution

The cell wall polysaccharides from Codium decorticatum and their assembly were studied and these results were compared with those obtained previously for this genus. The water soluble polysaccharides are: i) Pyruvylated and sulfated 3- and 6-linked B-D-galactans with sulfate mainly on C-4 and also on C-6. Pyruvate ketals are linked to O-3 and O-4 of terminal B-D-galactose or O-4 and O-6 of 3-linked B-D-galactose. ii) Sulfated 3-linked B-L-arabinans substituted on C-2 or C-2 and C-4 predominantly with sulfate, but also with single stubs of arabinose, and iii) 4-Linked B-D-mannans with a low degree of sulfation on C-2. The whole polysaccharide system comprises 6.9 % of sulfated polysaccharides and 32.9 % of fibrillar polysaccharides, mostly insoluble mannans. By in situ localization it was possible to detect two similar fibrillar layers separated by a zone rich in charged polymers. Besides, arabinogalactan proteins co-localized with the fibrillar components. Fil: Fernández, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Química de Biomoléculas; Argentina Fil: Raffo, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina