The Rumex vesicarius seeds water extract effect in the in vitro fertilization and embryo development of mice as mammalian model

ABSTRACT During IVF and embryo development in vitro there are a lot of free radical oxygen reactive spices (ROS), the lower the successful viable embryo rate. Rumex vesicarius plant is used as food and acts as a biochemical marker and as a scavenger of the free radical (ROS). The aim of this research was to study the role of Rumex vesicarius water seeds extract (RVWSE) on ova (IVF) and embryo development of mice. Water seed extracts of RV were made at a concentration of 10 mg/ml. These extracts were intended to be added to the fertilization medium of mice oocytes. This study showed that the RVWSE treated ova had showed significantly (P<0.05) higher rate of the in vitro fertilization and early cleavage development rate (2-4 cell) stage compared to control. Also, the rate of developed embryo from the cell stage to morula stage of the (RVWSE) treated group were higher (75.2%) compared to the control (62.65%.). Regarding the blastula stage, the control group showed significantly (P<0.05) higher developmental rate (26.35%) compared to the treated group (12.8%). The (10 mg/ml) dosage of supplementation of (RVWSE) in this research were significantly higher on the in vitro fertilization and early cleavage rate but not the blastula stage in mice.