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Cloning and sequencing of c-DNA encoding N-methyl-D-aspartate receptor subunit-1 in the hypothalamus of male sheep

Ana Zuley Ruiz · Sociedade Brasileira de Zootecnia · 2014

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Autore / responsabile
Ana Zuley Ruiz
Editore
Sociedade Brasileira de Zootecnia
Anno
2014
ISSN
1806-9290
ISSN
1806-9290
Lingua
Inglés
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The objective of the present study was to report the molecular cloning and determination of the sequences N-methyl-D-aspartate subunit-1 receptor (NMDAR-1) in the hypothalamus of sheep. The re-amplified DNA template for NMDA -1 from the hypothalamus of male sheep was cloned using pBluescript-Sk-plasmids (pBSK, 2958 bp). Purified plasmids containing the NMDA receptor c-DNA were sequenced using the dye-terminator chemistry at the University of Nebraska-Lincoln, Nebraska, USA. Results were entered into the National Center for Biotechnology Web site as accession number AY434689. The sequence of Ovis aries (sheep) NMDAR-1 mRNA from hypothalamus of male sheep has 94-97% homology with Homo sapiens, 94-100% homology with Sus scrofa, and 88-90% homology with Rattus norvegicus NMDAR-1 mRNA sequence, and 94-97% homology with Homo sapiens glutamate receptor and transcript variant NR1-2 and NR1-1 mRNA sequence. These results show high evolutionary conservation of NMDA receptor subunit-1 across species.

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APA 7

Ruiz, A. Z. (2014). Cloning and sequencing of c-DNA encoding N-methyl-D-aspartate receptor subunit-1 in the hypothalamus of male sheep. https://doi.org/10.1590/S1516-35982014000400004

MLA

Ruiz, Ana Zuley. "Cloning and sequencing of c-DNA encoding N-methyl-D-aspartate receptor subunit-1 in the hypothalamus of male sheep." 2014. https://doi.org/10.1590/S1516-35982014000400004.

Chicago

Ruiz, Ana Zuley. 2014. "Cloning and sequencing of c-DNA encoding N-methyl-D-aspartate receptor subunit-1 in the hypothalamus of male sheep.". https://doi.org/10.1590/S1516-35982014000400004.

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Ruiz, A. Z. 2014, Cloning and sequencing of c-DNA encoding N-methyl-D-aspartate receptor subunit-1 in the hypothalamus of male sheep, Sociedade Brasileira de Zootecnia, available at: https://doi.org/10.1590/S1516-35982014000400004 [Accessed 8 Aug. 2026].

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Titolo
Cloning and sequencing of c-DNA encoding N-methyl-D-aspartate receptor subunit-1 in the hypothalamus of male sheep
Autore / collaboratori
Ana Zuley Ruiz
Editore
Sociedade Brasileira de Zootecnia
Anno di pubblicazione
2014
ISSN
1806-9290
ISSN
1806-9290
Lingua
Inglés

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cloning; NMDA receptor; Ovis aries; ram; sequencing
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