Synthesis of biodegradable microspheres of tramadol by simple phase separation technique and their in vitro evaluation

Biodegradable microspheres of Tramadol Hydrochloride (TmH) were developed using simple
phase separation technique. Poly lactide-co-glycolide (PLGA) was employed as a release controlling
polymer. Simple phase separation method was adopted to prepare microspheres; Dichloromethane (DCM)
and Liquid Paraffin (LP) were employed as solvent and non-solvent respectively. Five kinetic models were
applied to assess and describe the mechanism and pattern of TmH release from biodegradable microspheres.
Biodegradable microspheres were subjected to FTIR, DSC and XRD, to evaluate TmH-PLGA interaction.
Retardation in the release of TmH was observed as PLGA concentration was increased. Kinetics
of drug release was following higuchi model. The microspheres exhibited no interaction between TmH and
PLGA. Biodegradable microspheres of TmH can be produced using phase separation method. Microspheres
were stable with no drug-polymer interaction. The accelerated stability studies also ensured the
physicochemical integrity as differences of release profile over the period of three months were insignificant.
Colegio de Farmacéuticos de la Provincia de Buenos Aires