Standardization of Caco-2 cell culture as in vitro model for intestinal permeability

Artículo

Standardization of Caco-2 cell culture as in vitro model for intestinal permeability

Rodríguez, Yanina I. et al · SEDICI UNLP · 2011

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Autore / responsabile

Rodríguez, Yanina I. et al

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SEDICI UNLP

Anno

2011

Lingua

Inglés

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The aim of this study was to find out the optimal experimental conditions for Caco-2 cell culture (time and density) and permeability assays (diffusion system and drug concentration) in order to study the in vitro drugs permeability as a predictive method for drug absorption across intestinal epithelium. The integrity of the monolayers used in each assay was determined by measuring the transepithelial electrical resistance (TEER) and the permeability of the atenolol-a drug which is transported across the monolayers by the paracellular pathway-. The best working condition was obtained with a cell seeding of 7.104 cells/insert in a vertical difussion chamber. In such context, the monolayers had a TEER higher than 550 Ω.cm2 and the apparent permeability coefficient of atenolol was 0.71 ± 0.19 x 10-6 cm/seg. Colegio de Farmacéuticos de la Provincia de Buenos Aires

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APA 7

Rodríguez, Y. I. E. A. (2011). Standardization of Caco-2 cell culture as in vitro model for intestinal permeability. http://sedici.unlp.edu.ar/handle/10915/8097

MLA

Rodríguez, Yanina I. et al. "Standardization of Caco-2 cell culture as in vitro model for intestinal permeability." 2011. http://sedici.unlp.edu.ar/handle/10915/8097.

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Rodríguez, Yanina I. et al. 2011. "Standardization of Caco-2 cell culture as in vitro model for intestinal permeability.". http://sedici.unlp.edu.ar/handle/10915/8097.

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Rodríguez, Y. I. E. A. 2011, Standardization of Caco-2 cell culture as in vitro model for intestinal permeability, SEDICI UNLP, available at: http://sedici.unlp.edu.ar/handle/10915/8097 [Accessed 7 Aug. 2026].

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Titolo: Standardization of Caco-2 cell culture as in vitro model for intestinal permeability

Autore / collaboratori: Rodríguez, Yanina I. et al

Editore: SEDICI UNLP

Anno di pubblicazione: 2011

Lingua: Inglés

Soggetti

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Farmacia; Técnicas de Cultivo de Célula; atenolol; biopharmaceutics classification system; Caco-2; permeability; Permeabilidad