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Assessment of experimental infection for dogs usingGallus gallus chorioallantoic membranes inoculated withNeospora caninum

Alexandre Dias Munhoz et al · Colégio Brasileiro de Parasitologia Veterinária

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The aim of this study was to evaluate parasitism kinetics and tissue lesions in the first week of infection by Neospora caninum in dogs fed Gallus gallus chorioallantoic membranes (CMs) previously infected in ovo. Five two-month-old pups were used. Each dog was given five CMs that were previously infected with N. caninum via the oral route. Four animals were euthanized in the first week of infection. All four dogs had their stools examined one week prior to and up to the day they were euthanized. The stools of the uneuthanized dog were collected for 30 days. After euthanasia, organ sections were utilized for histopathology, immunohistochemistry, indirect immunofluorescent tissue reactions, PCR and real-time PCR to detect parasites. Necropsy revealed that the small and large intestines, spleen, and lungs were affected. No oocysts orN. caninum DNA were identified in the stool samples. Real-time PCR was the most sensitive technique used to detect the protozoa in tissues, which were identified in 41% of the analyzed samples. Our results indicate that an experimental model using previously infected CMs appears to be a useful model for the study of the host-parasite relationship during the infection's acute phase.

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APA 7

al, A. D. M. E. (s. f.). Assessment of experimental infection for dogs usingGallus gallus chorioallantoic membranes inoculated withNeospora caninum. https://doi.org/10.1590/S1984-29612013000400018

MLA

al, Alexandre Dias Munhoz et. "Assessment of experimental infection for dogs usingGallus gallus chorioallantoic membranes inoculated withNeospora caninum.". https://doi.org/10.1590/S1984-29612013000400018.

Chicago

al, Alexandre Dias Munhoz et. s. f. "Assessment of experimental infection for dogs usingGallus gallus chorioallantoic membranes inoculated withNeospora caninum.". https://doi.org/10.1590/S1984-29612013000400018.

Harvard

al, A. D. M. E. s. f, Assessment of experimental infection for dogs usingGallus gallus chorioallantoic membranes inoculated withNeospora caninum, Colégio Brasileiro de Parasitologia Veterinária, available at: https://doi.org/10.1590/S1984-29612013000400018 [Accessed 28 Jun. 2026].

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Título
Assessment of experimental infection for dogs usingGallus gallus chorioallantoic membranes inoculated withNeospora caninum
Autor / colaboradores
Alexandre Dias Munhoz et al
Editorial
Colégio Brasileiro de Parasitologia Veterinária
ISSN
1984-2961
ISSN
1984-2961
Idioma
eng

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