Production of plants from callus of Vigna radiata stem seedlings

Abstract <br /> The present study include callus initiation from leaves and stems explants of Vigna radiata seedlings by using solidified Murashige and Skoog (MS) medium supplemented with different concentrations of Naphthalene acetic acide NAA (0.5,1.0,2.0)mg/L and benzyl adenin BA (0.1,0.2,0.3,0.5,1.0,2.0)mg/L. The results showed that Ms medium which contains 2.0 mg/l of NAA was the best one for callus initiation from leaves while solidified MS medium containing 0.5 mg/L NAA and 2.0 mg/L BA was superior for callus induction from stem. The callus produced from stems and leaves has been maintained on solidified MS medium supplemented with 0.2 mg/L Kin and 0.2 mg/L 2,4-D for shoot regeneration, MS media containing 0.5mg/L NAA and different concentration of BA (0.5, 1.0, 2.0, 3.0, 4.0, and 5.0)mg/L were used. The results indicated that MS medium with 5.0 mg/L BA was the best one for shoot regeneration. Moreover, ther was a difficulty in rooting the regenerated shoots, Since they failed to produce roots in the rooting media which were used except solidified MS medium supplemented with 2mg/L NAA.