First report of phleboviruses in phlebotomine sandfly communities from northern Colombia

The genus Phlebovirus is considered a medically important group of viruses due to the pathogenic behavior of some species. In the Americas, several species of this genus have been identified, most of which are found in Panamá. However, the lack of knowledge about the circulation of phleboviruses in Colombia led us to ask several questions, including the distribution of this pathogen in the Americas, especially in Caribbean coastal areas where ideal conditions for phlebovirus vectors exist. Here, we report the first detection of the genus Phlebovirus in phlebotomine sandfly communities from the Caribbean coast of Colombia. Sandflies were collected and pooled for ribonucleic acid (RNA) extraction and retrotranscription, all pools were tested for 18S ribosomal RNA and screened for phleboviruses by nested reverse transcription-polymerase chain reaction (RT-PCR). Positive products for the RNA-dependent RNA polymerase (RdRp) detection were sequenced. Four pools of 58 were positive for viruses belonging to the Punta Toro complex, Aguacate complex, Old-World viruses, and one related to sloth phleboviruses. This is the first evidence of phleboviruses in the Caribbean region of Colombia, especially of species related to the Punta Toro and Aguacate complex.