Gas exchange in massai grass under five nitrogen fertilization levels during establishment and regrowth

The objective of this study was to evaluate gas exchange in the massai grass during establishment and regrowth cycles in a greenhouse, using five nitrogen (N) fertilization levels. A completely randomized split-plot design of five nitrogen levels (0; 150; 300; 450 and 600 mg N•dm-3 of soil) was employed with the N levels as plots, and the cycles (establishment, regrowth 1 and 2) as subplots, evaluated with five replicates. We evaluated leaf transpiration (E), leaf temperature (TFOL), leaf photosynthesis rate (A), leaf carbon dioxide concentration (Ci), stomatal conductance (gs), photosynthesis/transpiration ratio (A/E), photosynthesis/conductance ratio (A/gs), chlorophyll relative index (CRI), and nitrogen sufficiency index (NSI). Growth cycles influenced all physiological characteristics, except for NSI. N fertilization levels affected TFOL, A, CRI and NSI variables, whereas for gs, E, Ci, A/E and A/gs it did not occur. A increased from 6.38 to 10.33 µmol•m-2.s-1, at levels 0 and 600 mg N•dm-3 of soil, respectively. Gas exchange in the massai grass was favored by nitrogen fertilization, since the cycles amended these characteristics of the grass.