Analysis of Blood Serum and Gallstone Lipids Composition of Gallstone Patients

ABSTRACT<br /> This study was designed to compare the level of lipid fractions and percentage of fatty acids in serum and gallstones in patients with gallstone, the study include 25 gallstone patients who were diagnosed by ultrasonography,the age is between (40-60) year and compared with (25) normal subject with same age were collected as control and measurement of a number of biochemical parameters in serum and gallstone,as well as analysis and measurement of percentage of fatty acids in the fatty component of serum and gallstone (cholesterol ester, phospholipids and triglyceride) by using thin layer chromatography (TLC) and then re-esterfication fatty acids and measurement percentage of fatty acids using capillary gas chromatography (CGC). also the study include chemical composition of gallstones by using Fourier transform infrared (FTIR). the result of this study show that there is a significant differences in the level of studied biochemical parameter and fatty acids percentage in gallstone patients compared with the control group.