In vitro evaluation of the effect of ozonated olive oil containing dentifrice on enamel color change and microhardness

Background/Aim: Nowadays, people have an insatiable desire for white and beautiful teeth. The present in vitro study aimed to evaluate the effect of experimental toothpaste containing ozonated olive oil commercial whitening toothpaste and ozone-free olive oil toothpaste, on color change and enamel microhardness. Material and Methods: In this in vitro study, 48 bovine incisors were mounted in an acrylic resin. After the immersion of samples in tea solution, each sample was subjected to the Vickers microhardness test and colorimetry (T1). The samples were then randomly assigned to four groups (n=12) and brushed twice a day for two min with an electric toothbrush with toothpaste specific to each group; Group 1: commercial whitening toothpaste (positive control), Group 2: Experimental toothpaste containing ozone-free olive oil , Group 3: Experimental 10 mg/ml ozonated olive oil toothpaste, Group 4: Experimental toothpaste containing ozonated olive oil with an ozone concentration of 15 mg/ml. Surface microhardness measurements and color evaluation were repeated after two (T2) and four weeks (T3). Data were analyzed by, repeated measures analysis of variance, Friedman, ANOVA, and Kruskal-Wallis. Results: The highest mean of color change (DE) was related to toothpaste containing 15 mg/ml ozonated olive oil, and the lowest mean pertained to toothpaste containing ozone-free olive oil (P=0/019). In these four groups, the amount of microhardness increased after the end of the study (P=0.001); nonetheless, these changes were not significant. Conclusions: The addition of ozone to toothpaste in a specific concentration results in teeth whitening; however, it does not significantly affect enamel microhardness.