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Accuracy of quantitative polymerase chain reaction in samples of frozen and paraffin-embedded healthy skin for the diagnosis of canine visceral leishmaniasis

M.P. Campos et al · Universidade Federal de Minas Gerais, Escola de Veterinária

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Autor / responsable
M.P. Campos et al
Editorial
Universidade Federal de Minas Gerais, Escola de Veterinária
ISSN
1678-4162
ISSN
1678-4162
Idioma
Inglés
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ABSTRACT The purpose of the present work was to evaluate the accuracy of quantitative polymerase chain reaction (qPCR) performed on samples of fresh frozen tissue (FT) and formalin-fixed, paraffin-embedded (FFPE) healthy skin. This is a validation study conducted with samples from 46 dogs from an endemic area in Brazil. After sample collection, DNA extractions were conducted using commercial kits and qPCR was oriented to kinetoplast DNA (kDNA) targets of the Leishmania infantum species. The results obtained for the FFPE samples showed 63.6% sensitivity and 77.1% specificity, whereas those obtained for the FT samples showed 100% and 48.6%, respectively. Poor agreement was observed for the results of the qPCR technique with FT and FFPE samples. Our results suggest freezing as the most suitable conservation method for the formation of sample databases considering DNA recovery

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APA 7

al, M. C. E. (s. f.). Accuracy of quantitative polymerase chain reaction in samples of frozen and paraffin-embedded healthy skin for the diagnosis of canine visceral leishmaniasis. https://doi.org/10.1590/1678-4162-9053

MLA

al, M.P. Campos et. "Accuracy of quantitative polymerase chain reaction in samples of frozen and paraffin-embedded healthy skin for the diagnosis of canine visceral leishmaniasis.". https://doi.org/10.1590/1678-4162-9053.

Chicago

al, M.P. Campos et. s. f. "Accuracy of quantitative polymerase chain reaction in samples of frozen and paraffin-embedded healthy skin for the diagnosis of canine visceral leishmaniasis.". https://doi.org/10.1590/1678-4162-9053.

Harvard

al, M. C. E. s. f, Accuracy of quantitative polymerase chain reaction in samples of frozen and paraffin-embedded healthy skin for the diagnosis of canine visceral leishmaniasis, Universidade Federal de Minas Gerais, Escola de Veterinária, available at: https://doi.org/10.1590/1678-4162-9053 [Accessed 8 Aug. 2026].

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Título
Accuracy of quantitative polymerase chain reaction in samples of frozen and paraffin-embedded healthy skin for the diagnosis of canine visceral leishmaniasis
Autor / colaboradores
M.P. Campos et al
Editorial
Universidade Federal de Minas Gerais, Escola de Veterinária
ISSN
1678-4162
ISSN
1678-4162
Idioma
Inglés

Materias

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diagnóstico; qPCR; leishmaniose visceral canina
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