Analiza stężeń eotaksyny 1/CCL11, eotaksyny 2/CCL24 i eotaksyny 3/CCL26 w surowicy dorosłych chorych na atopowe zapalenie skóry

Introduction. Eosinophils play an important role in development ofallergic diseases. Recent studies indicate their many relevant functionsin the development of allergic inflammation related to both an effectorand immunomodulatory role. The recognition of factors responsible foreosinophil recruitment to tissues may be significant for understandingallergic diseases’ regulatory processes, including mechanisms leadingto skin changes in atopic dermatitis (AD). The most prominent chemotacticfactors influencing eosinophil accumulation are those actingselectively via the CCR3 eotaxin receptor. Many reports indicate a relevantrole of these chemokines in pathogenesis of allergic diseases.Objective. To measure the level of serum concentrations of eotaxin1/CCL11, eotaxin 2/CCL24 and eotaxin 3/CCL26 in AD patients andhealthy subjects in relation to disease intensity assessed by the SCORADscale.Material and methods. Hundred and one AD patients and 21 healthysubjects as the control group were investigated. The concentrations ofeotaxin 1/CCL11, eotaxin 2/CCL24 and eotaxin 3/CCL26 were measuredusing enzyme-linked immunosorbent assay (ELISA) with R&DSystems kits (USA).Results. It was found that in the group of AD patients the average serumconcentration of 1/CCL11 eotaxin was 123.86 pg/ml. The average serumconcentration of 2/CCL24 eotaxin was 1064.52 pg/ml and of 3/CCL26eotaxin 16.93 pg/ml. In healthy volunteers (control group), the meanserum concentration of 1/CCL11 eotaxin was 195.64 pg/ml, the meanserum concentration of 2/CCL24 eotaxin was 965.58 pg/ml, and of3/CCL26 eotaxin 0.79 pg/ml. Comparative analysis of serum eotaxin levelsbetween the group of AD patients and the healthy controls showedthat the mean serum concentration of 1/CCL11 eotaxin in patients withatopic dermatitis is significantly lower (p = 0.002), and of 3/CCL26 eotaxinhigher (p = 0.0000001) than in healthy controls. In the group of ADpatients the average severity of illness rated with the SCORAD scale was53.17. Regression analysis between the exacerbation of disease rated withthe SCORAD scale and serum levels of 1/CCL11 eotaxin, 2/CCL24 eotaxinand 3/CCL26 eotaxin showed a statistically significant correlation forall measured parameters (respectively p = 0.001, p = 0.01, p = 0.00001).Conclusions. The analysis of results showed that in the researchedgroup: 1. Mean eotaxin 3/CCL26 and eotaxin 2/CCL24 serum concentrationis higher in AD patients as compared to healthy subjects and inthe case of eotaxin 3/CCL26 the difference is statistically significant.2. Mean eotaxin 1/CCL11 serum concentration is statistically lower inAD patients as compared to healthy subjects. 3. Eotaxin 1/CCL11,eotaxin 2/CCL24 and eotaxin 3/CCL26 serum concentrations correlatewith AD intensity assessed by SCORAD.