Seasonal variability of water parameters and construction of laccase biosensors using ureasil polymers for analysis of water pollutions

Physical and chemical indicators of underground waters of the Skole region were studied. The samples were taken from natural water sources of Skole district of Lviv region and analyzed within organoleptic and physico-chemical indicators of the quality of water. The organoleptic and physico-chemical indicators of the quality of natural water sources of the studied area meet the requirements of the State sanitary norms and rules. Organic-inorganic ureasil polymers with different precursors were used as holding matrixes in construction of laccase biosensors for analysis of water pollution. Cyclic voltammetry and chronoamperometric measurements were carried out to estimate the main parameters of the constructed biosensors. It is found that the sensitivity of the constructed biosensors correlates well with the network properties (free volume and crosslinking) of the investigated polymers studied using positron annihilation lifetime spectroscopy and swelling measurements. The same correlation was also earlier reported for ureasil composites of different prehistory and photopolymers and it seems to be universal that allows controlled biosensing according to the required needs of analysis.