Ozone therapy as a potential enhancer of anti-measles and anti-mumps virus antibodies in goats for hyperimmune serum production: a pilot study

ABSTRACT Goats have been proposed for hyperimmune serum production (HSP) in vaccine quality control, and ozone therapy may enhance immunoglobulin levels. This study aimed to establish an HSP protocol and evaluate whether major ozone autohemotherapy (O3-MAHT) could increase antibody titers in goats. A 2×2 crossover design was used, involving 10 goats inoculated with attenuated mumps (n=5) or measles (n=5) antigens. Twelve hours after booster inoculation, 240mL of whole blood were collected into bags, which either received 240mL of an O2/O3 gas mixture (20μg/mL; Gozone; n=5) or remained untreated (Gcontrol; n=5) before reinfusion. TBARS, MDA, complete blood count, and anti-measles and anti-mumps antibody titers were analyzed. A time effect was observed for TBARS, MDA, HCT, TPP, WBC, lymphocyte, segmented neutrophil, and monocyte indices. Antibody titers were affected by the study phase. In phase 1, there was a trend for time, treatment, and time × treatment interaction effects on viral particle inactivation. Additionally, Gozone tended to show greater viral particle inactivation at T21 compared to Gcontrol. All goats responded to the HSP protocol, and O3-MAHT showed a potential trend toward improving viral particle inactivation, supporting further investigation.