Buscar recursos académicos

Materias / palabras clave: microRNA; Biology; Gene expression profiling; Computational biology; RNA; Messenger RNA; Gene expression; Genetics; Gene; Bioinformatics

Materias / palabras clave: RNA-Seq; Biology; Transcriptome; Computational biology; RNA; DNA sequencing; Deep sequencing; De novo transcriptome assembly; Genetics; Gene; Genome; Gene expression

The fine detail provided by sequencing-based transcriptome surveys suggests that RNA-seq is likely to become the platform of choice for interrogating steady state RNA. In order to discover biologically important changes ...

limma is an R/Bioconductor software package that provides an integrated solution for analysing data from gene expression experiments. It contains rich features for handling complex experimental designs and for informatio...

Materias / palabras clave: RNA-Seq; Computational biology; Biology; Computer science; Gene; Transcriptome; splice; Reference genome; RNA splicing; Software; Genome; RNA

In comparative high-throughput sequencing assays, a fundamental task is the analysis of count data, such as read counts per gene in RNA-seq, for evidence of systematic changes across experimental conditions. Small replic...

A high-capacity system was developed to monitor the expression of many genes in parallel. Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measureme...

We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been...

BACKGROUND: RNA-Seq is revolutionizing the way transcript abundances are measured. A key challenge in transcript quantification from RNA-Seq data is the handling of reads that map to multiple genes or isoforms. This issu...

Materias / palabras clave: Exon; splice; Biology; Gene; RNA; Transcriptome; RNA-Seq; Genetics; Gene expression; Alternative splicing; RNA splicing; Computational biology

Materias / palabras clave: Enumeration; Biology; Haematopoiesis; RNA; Gene; Computational biology; Hematopoietic cell; Cell; Gene expression; Molecular biology; Messenger RNA; Cell biology

Materias / palabras clave: Biology; RNA-Seq; Gene; RNA splicing; Transcriptome; Computational biology; Alternative splicing; Genetics; Genome; De novo transcriptome assembly; Gene expression; Gene isoform

Although genomewide RNA expression analysis has become a routine tool in biomedical research, extracting biological insight from such information remains a major challenge. Here, we describe a powerful analytical method ...

Tremendous amount of RNA sequencing data have been produced by large consortium projects such as TCGA and GTEx, creating new opportunities for data mining and deeper understanding of gene functions. While certain existin...

BACKGROUND: Gene set enrichment (GSE) analysis is a popular framework for condensing information from gene expression profiles into a pathway or signature summary. The strengths of this approach over single gene analysis...

Infiltrating stromal and immune cells form the major fraction of normal cells in tumour tissue and not only perturb the tumour signal in molecular studies but also have an important role in cancer biology. Here we descri...

MOTIVATION: A new protocol for sequencing the messenger RNA in a cell, known as RNA-Seq, generates millions of short sequence fragments in a single run. These fragments, or 'reads', can be used to measure levels of gene ...

The problem of identifying differentially expressed genes in designed microarray experiments is considered. Lonnstedt and Speed (2002) derived an expression for the posterior odds of differential expression in a replicat...

We analysed primary breast cancers by genomic DNA copy number arrays, DNA methylation, exome sequencing, messenger RNA arrays, microRNA sequencing and reverse-phase protein arrays. Our ability to integrate information ac...

Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to becoming a routine tool in molecular biology to study low abundance gene expression. Real-time ...

To characterize somatic alterations in colorectal carcinoma, we conducted a genome-scale analysis of 276 samples, analysing exome sequence, DNA copy number, promoter methylation and messenger RNA and microRNA expression....