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This work describes a new method for extracting genomic DNA from heavy oil-contaminated soils. This method combines soil washes using three washing solutions with enzymatic lysis (SW-EL method). The suitability of the SW...

The chemically synthesizable quantum emitters such as quantum dots (QDs), fluorescent nanodiamonds (FNDs), and organic fluorescent dyes can be integrated with an easy-to-craft quantum nanophotonic device, which would be ...

Flow cytometric analysis of DNA content was performed on 28 samples of canine mammary tumors. Nine of them were benign and 19 were malignant. All benign tumors and 11 malignant tumors (57.9%) were diploid (P

Summary This study was designed to evaluate the quality and viability of bovine embryos produced by in vitro fertilization (IVF), after intracytoplasmic injection of pCX-EGFP-liposome complexes or pBCKIP2.8-liposome comp...

Objective: Sleep deprivation and poor quality of sleep are a current issue in our society. In animal models, sleep deprivation can induce diverse alterations, including oxidative stress in several organs. A highly active...

Abstract Background As dementia cases continue to rise, effective prevention strategies are urgently needed. However, objective biomarkers that directly reflect lifestyle factors remain limited. Life’s Essential 8 (LE8...

Abstract Background Epigenetic mechanisms including DNA methylation are assumed to play crucial roles in the maintenance of genome integrity, regulation of gene expression and development, and their increasing exploitati...

The development of a novel microfluidic device that includes a circular microchannel and integrated electrodes for DNA electrophoresis is reported. The geometry of the separation channel and the arrangement of the embedd...

Abstract Purpose Patients with nasopharyngeal carcinoma (NPC) exhibiting persistent EBV DNA positivity throughout treatment (a “D-D-D” trajectory) represent a high-risk group with undefined management strategies. Thi...

Seaweeds hold immense economic potential due to multifarious applications in pharmaceuticals and everyday products. India’s 11,099 km coastline harbours a rich diversity of seaweeds in the Indian Ocean. Identifying sea...

Abstract Background Cervical cancer remains the fourth most common malignancy among women worldwide, with limited therapeutic success in advanced and recurrent stages. Conventional treatments, including radiotherapy and ...

BackgroundThe methylation status of cell-free DNA (cfDNA) shows promise for the clinical detection of cervical cancer and its precursors; however, its measurement in liquid biopsies is hindered by low cfDNA yields and te...

BackgroundAlterations in ribosomal DNA copy number (rDNA-CN) and global DNA methylation have been associated with genomic instability and various diseases. However, their relevance in myocardial infarction (MI) has not b...

Abstract Therapeutic human papillomavirus (HPV) DNA vaccine is an attractive option to control existed HPV infection and related lesions. The two early viral oncoproteins, E6 and E7, are continuously expressed in most HP...

We have analyzed the DNA-binding properties of the complex formed by the Arabidopsis TALE homeodomain (HD) proteins STM and BLH3 in comparison with those of the individual proteins. In vitro DNA-binding assays indicated ...

Aberrant DNA methylation observed in cancer can provide survival benefits to cells by silencing genes essential for anti-tumor activity. DNA-demethylating agents such as Decitabine (DAC)/Azacitidine (AZA) activate otherw...

The use of long ssDNA polynucleotides in PCR of degraded DNA samples can discriminate Short Tandem Repeat (STR) genotypes otherwise missed by current commercial kits. Fil: Mautner, Martin E.. Biodynamics; Argentina Fil: ...

Abstract Simultaneous inhibition of DNA gyrase and topoisomerase IV (Topo IV) is a primary pharmacological strategy to enhance antibacterial efficacy and markedly reduce the emergence of antibiotic resistance. In this re...

OBJETIVO: avaliar um novo método de determinação do sexo fetal pela análise de DNA obtido do plasma materno. MÉTODOS: sangue periférico (10 mL) foi coletado de mulheres grávidas em diferentes idades gestacionais. ...

Abstract Background Sudden Infant Death Syndrome (SIDS) remains a leading cause of infant mortality worldwide, yet its underlying mechanisms are largely unknown. SIDS etiology is complex and involves the interplay betwee...

Background and Aim: Cryopreservation of boar semen is widely used for genetic conservation and artificial insemination; however, it induces oxidative stress and molecular alterations that compromise sperm function. Epige...

Abstract Effective management decisions rely on accurate insights into species’ population structure, with high-quality genetic material being essential. Stranded cetaceans are an important source of samples, but meeti...

Protein recognition of DNA sites is a primary event for gene function. Its ultimate mechanistic understanding requires an integrated structural, dynamic, kinetic, and thermodynamic dissection that is currently limited co...

Foram testados três métodos de extração de DNA em amostras de queijo, com o objetivo de identificar uma técnica eficiente para extração de DNA em amostras com várias limitações, como alto teor de gordura, alto ...