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Materias / palabras clave: Science: Biology (General): Genetics; Science: Microbiology

BackgroundEuschistus heros and Piezodorus guildinii are major soybean pests across the Americas. Gut-associated bacteria influence insect nutrition, detoxification, and stress tolerance, potentially enhancing adaptation ...

Materias / palabras clave: Science: Microbiology; Science: Biology (General): Genetics

Abstract Background The complex relationship between the gut microbiome and immune system development during infancy is considered a key factor in the rising rates of pediatric allergic diseases. Food protein-induced all...

The use of antibiotic growth promoters (AGPs) in poultry production has raised concerns for human, animal, and environmental health owing to the development and spread of antimicrobial resistance. These concerns have tri...

BackgroundThe research on human microbiota-associated (HMA) animal models is an important tool for studying the human microbiome and holds great potential for elucidating disease mechanisms and microbe-based therapeutic ...

The coevolution of the human diet and gut microbiome has played a pivotal role in shaping metabolic, immune, and epigenetic functions across human history. Dietary transitions from high-fiber ancestral patterns to modern...

ABSTRACT Cystic fibrosis (CF) and inflammatory bowel disease (IBD) represent distinct pathologies with unique genetic underpinnings; yet, they share remarkable similarities in gut microbiota dysbiosis and intestinal phys...

The rhizosphere is one of the most dynamic biological environments on Earth. Within this narrow zone surrounding plant roots, plants interact constantly with diverse communities of microorganisms including bacteria, fung...

Abstract Studying microbial biodiversity offers enormous potential for improving the valorisation of plant biomass, particularly in conversion processes. Microorganisms, such as bacteria, fungi, yeasts and algae, play an...

To understand the impact of gut microbes on human health and well-being it is crucial to assess their genetic potential. Here we describe the Illumina-based metagenomic sequencing, assembly and characterization of 3.3 mi...

We describe a new molecular approach to analyzing the genetic diversity of complex microbial populations. This technique is based on the separation of polymerase chain reaction-amplified fragments of genes coding for 16S...

DNA sequencing continues to decrease in cost with the Illumina HiSeq2000 generating up to 600 Gb of paired-end 100 base reads in a ten-day run. Here we present a protocol for community amplicon sequencing on the HiSeq200...

mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package...

Advances in modern sequencing technologies allow us to generate sufficient data to analyze hundreds of bacterial genomes from a single machine in a single day. This potential for sequencing massive numbers of genomes cal...

Materias / palabras clave: Metagenomics; Biology; Phylogenetic tree; 16S ribosomal RNA; Gene; Computational biology; Phylogenetics; Microbiome; Genetics; Ribosomal RNA; Genome; Evolutionary biology

Materias / palabras clave: Operational taxonomic unit; Amplicon; Biology; Computational biology; Amplicon sequencing; Pipeline (software); Genetics; Gene; Computer science; Polymerase chain reaction; 16S ribosomal RNA; Programming language

Fil: Faccone, Diego. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina. Streptococcus pneumo...

Studies of the human microbiome have revealed that even healthy individuals differ remarkably in the microbes that occupy habitats such as the gut, skin and vagina. Much of this diversity remains unexplained, although di...

16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The accuracy of these analyses depends strongly on the choice of primers...

The ongoing revolution in high-throughput sequencing continues to democratize the ability of small groups of investigators to map the microbial component of the biosphere. In particular, the coevolution of new sequencing...

This study describes and validates a new method for metagenomic biomarker discovery by way of class comparison, tests of biological consistency and effect size estimation. This addresses the challenge of finding organism...

The Ribosomal Database Project (RDP) Classifier, a naïve Bayesian classifier, can rapidly and accurately classify bacterial 16S rRNA sequences into the new higher-order taxonomy proposed in Bergey's Taxonomic Outline of...

BACKGROUND: the analysis of microbial communities through dna sequencing brings many challenges: the integration of different types of data with methods from ecology, genetics, phylogenetics, multivariate statistics, vis...