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Background: This study developed and systematically optimized an ion-pair hydrophilic interaction liquid chromatography (IP-HILIC) system for oligonucleotide medicine analysis, aiming to the issues of insufficient resolu...

O presente artigo descreve a influência da adição de quantidades crescentes do lipídeo catiônico estearilamina (SA) e da presença do tensoativo não-iônico poloxâmero 188 sobre características físicoquímicas d...

In this paper we report exploratory analyses of high-density oligonucleotide array data from the Affymetrix GeneChip system with the objective of improving upon currently used measures of gene expression. Our analyses ma...

Elevated lipoprotein(a) [Lp(a)] is a genetically determined and independent risk factor for atherosclerotic cardiovascular disease (ASCVD) that is largely resistant to conventional lipid-lowering therapies. Novel Lp(a)-t...

The therapeutic benefit of phosphorothioate oligodeoxynucleotides (PS-ODN) containing immune stimulatory sequences has been demonstrated in animal models of cancer and infection. Several tools are available for the deter...

Abstract C4ʹ-modified nucleoside analogues continue to attract global attention for their use in antiviral drug development and oligonucleotide-based therapeutics. However, current approaches to C4ʹ-modified nucleoside...

OBJETIVO: avaliar um novo método de determinação do sexo fetal pela análise de DNA obtido do plasma materno. MÉTODOS: sangue periférico (10 mL) foi coletado de mulheres grávidas em diferentes idades gestacionais. ...

Determinou-se a expressão gênica das caspases 3 e 8 mediante transcrição reversa de mRNA total e reação em cadeia da polimerase (RT-PCR) para avaliar a apoptose em timo e baço de ratas imunossuprimidas por glicoco...

O objetivo deste trabalho foi desenvolver uma PCR em tempo real (qPCR) para o diagnóstico rápido e sensível da doença de Aujeszky. Os iniciadores amplificaram um fragmento de 123 pares de base do gene codificante da ...

Natural antisense transcripts (NATs) from eukaryotic genes, known as long non-coding RNAs (lncRNAs), are long transcripts that do not encode proteins. NATs play diverse functional roles in regulating the transcription, s...

Abstract Autism spectrum disorder (ASD) is a neurodevelopmental condition characterized by heterogeneity in its genetic, epigenetic, and environmental etiologies, posing significant challenges for therapeutic development...

IntroductionMacrophages play a key role in inflammatory diseases. We aimed to identify long noncoding RNAs that regulate pro-inflammatory activation of human macrophages. MethodsWe performed human lncRNA microarray analy...

Two new methods were used to establish a rapid and highly sensitive prenatal diagnostic test for sickle cell anemia. The first involves the primer-mediated enzymatic amplification of specific beta-globin target sequences...

Materias / palabras clave: Endonuclease; DNA; Restriction enzyme; Agarose; Oligonucleotide; Chemistry; Molecular biology; DNA polymerase; DNA clamp; Polymerase; Polymerase chain reaction; Biochemistry

A set of oligonucleotide primers capable of initiating enzymatic amplification (polymerase chain reaction) on a phylogenetically and taxonomically wide range of bacteria is described along with methods for their use and ...

A novel DNA fingerprinting technique called AFLP is described. The AFLP technique is based on the selective PCR amplification of restriction fragments from a total digest of genomic DNA. The technique involves three step...

We describe a new molecular approach to analyzing the genetic diversity of complex microbial populations. This technique is based on the separation of polymerase chain reaction-amplified fragments of genes coding for 16S...

Materias / palabras clave: nanotechnology, pharmaceutical sciences, oligonucleotide, drugs, pharmacology; Medicine: Medicine (General)