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Saliva direct PCR protocol for HLA‐DQB1*02 genotyping

Carrillo, Angeles et al · Wiley Blackwell Publishing, Inc · 2024

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Author / responsible party
Carrillo, Angeles et al
Publisher
Wiley Blackwell Publishing, Inc
Year
2024
ISSN
1744-3121
ISSN
1744-3121
Language
English

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Celiac disease (CD) is an immune disorder, that is triggered by gluten ingestion in genetically predisposed individuals. The HLA-DQB1*02 allele is the main predisposing genetic factor and a candidate for first-line genotyping screening. We designed and validated a simple, DNA purification-free PCR protocol directly from crude saliva, enabling the detection of the DQB1*02 allele. This assay also distinguishes homozygous from heterozygous carriers. We propose this method for use in mass screening and/or epidemiological studies. Fil: Carrillo, Angeles. Universidad Nacional de San Luis; Argentina Fil: Manzur, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina

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APA 7

Carrillo, A. E. A. (2024). Saliva direct PCR protocol for HLA‐DQB1*02 genotyping. http://hdl.handle.net/11336/257195

MLA

Carrillo, Angeles et al. "Saliva direct PCR protocol for HLA‐DQB1*02 genotyping." 2024. http://hdl.handle.net/11336/257195.

Chicago

Carrillo, Angeles et al. 2024. "Saliva direct PCR protocol for HLA‐DQB1*02 genotyping.". http://hdl.handle.net/11336/257195.

Harvard

Carrillo, A. E. A. 2024, Saliva direct PCR protocol for HLA‐DQB1*02 genotyping, Wiley Blackwell Publishing, Inc, available at: http://hdl.handle.net/11336/257195 [Accessed 10 Aug. 2026].

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Title
Saliva direct PCR protocol for HLA‐DQB1*02 genotyping
Author / contributors
Carrillo, Angeles et al
Publisher
Wiley Blackwell Publishing, Inc
Publication year
2024
ISSN
1744-3121
ISSN
1744-3121
Language
English

Subjects

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autoimmune; disease; human leucocyte antigen; immunogenetics; https://purl.org/becyt/ford/1.6; https://purl.org/becyt/ford/1
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