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DNA sequencing with chain-terminating inhibitors

Frederick Sanger; S. Nicklen; Alan Coulson · Proceedings of the National Academy of Sciences · 1977

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Author / responsible party
Frederick Sanger; S. Nicklen; Alan Coulson
Publisher
Proceedings of the National Academy of Sciences
Year
1977
Language
English

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A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2',3'-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage varphiX174 and is more rapid and more accurate than either the plus or the minus method.

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APA 7

Sanger, F, Nicklen, S, & Coulson, A. (1977). DNA sequencing with chain-terminating inhibitors. https://doi.org/10.1073/pnas.74.12.5463

MLA

Sanger, Frederick, et al. "DNA sequencing with chain-terminating inhibitors." 1977. https://doi.org/10.1073/pnas.74.12.5463.

Chicago

Sanger, Frederick, S. Nicklen, and Alan Coulson. 1977. "DNA sequencing with chain-terminating inhibitors.". https://doi.org/10.1073/pnas.74.12.5463.

Harvard

Sanger, F, Nicklen, S. and Coulson, A. 1977, DNA sequencing with chain-terminating inhibitors, Proceedings of the National Academy of Sciences, available at: https://doi.org/10.1073/pnas.74.12.5463 [Accessed 7 Aug. 2026].

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Resource details

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Title
DNA sequencing with chain-terminating inhibitors
Author / contributors
Frederick Sanger; S. Nicklen; Alan Coulson
Publisher
Proceedings of the National Academy of Sciences
Publication year
1977
Language
English

Subjects

Explore related resources through these subjects.

DNA; Sanger sequencing; Nucleotide; DNA polymerase; Polymerase chain reaction; Chemistry; DNA sequencing; Bacteriophage; Polymerase; Molecular biology; Biology; Biochemistry
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