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BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs

Felipe A. Simão; Robert M. Waterhouse; Panagiotis Ioannidis; Evgenia V. Kriventseva; Evgeny M. Zdobnov · Bioinformatics · 2015

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Author / responsible party
Felipe A. Simão; Robert M. Waterhouse; Panagiotis Ioannidis; Evgenia V. Kriventseva; Evgeny M. Zdobnov
Publisher
Bioinformatics
Year
2015
Language
English

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MOTIVATION: Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50. RESULTS: We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO. AVAILABILITY AND IMPLEMENTATION: Software implemented in Python and datasets available for download from http://busco.ezlab.org. CONTACT: evgeny.zdobnov@unige.ch SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

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APA 7

Simão, F. A, Waterhouse, R. M, Ioannidis, P, Kriventseva, E. V, & Zdobnov, E. M. (2015). BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs. https://doi.org/10.1093/bioinformatics/btv351

MLA

Simão, Felipe A, et al. "BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs." 2015. https://doi.org/10.1093/bioinformatics/btv351.

Chicago

Simão, Felipe A, Robert M. Waterhouse, Panagiotis Ioannidis, Evgenia V. Kriventseva, and Evgeny M. Zdobnov. 2015. "BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs.". https://doi.org/10.1093/bioinformatics/btv351.

Harvard

Simão, F. A. et al. 2015, BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs, Bioinformatics, available at: https://doi.org/10.1093/bioinformatics/btv351 [Accessed 7 Aug. 2026].

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Title
BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs
Author / contributors
Felipe A. Simão; Robert M. Waterhouse; Panagiotis Ioannidis; Evgenia V. Kriventseva; Evgeny M. Zdobnov
Publisher
Bioinformatics
Publication year
2015
Language
English

Subjects

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Python (programming language); Annotation; Benchmarking; Computer science; Software; Genome; Genomics; Gene Annotation; Computational biology; Download; Biology; Gene
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