News Help Institutional access
Back to results
Bibliographic record · Consultation and access
Artículo

Driving forces for the adsorption of a His-tag Chagas antigen: a rational approach to design bio-functional surfaces

Valenti, Laura Elisa et al · Elsevier Science · 2013

Open-access full text
Quick overview. Review the resource’s basic details, then access the content using the main button. This page shows only the information needed to identify, cite, and open the work.
Author / responsible party
Valenti, Laura Elisa et al
Publisher
Elsevier Science
Year
2013
ISSN
0927-7765
ISSN
0927-7765
Language
English

Resource access

Open the content from the main option or choose another available source.

CONICET Digital CONICET Digital OAI-PMH
Entrar por CONICET Digital
Main access

Open-access full text

Texto completo identificado como acceso abierto.
Open text

Summary

Descripción general del contenido del recurso.

In order to rationally design a bio-functional surface based on the adsorption of a His-tag antigen, three requirements have to be considered: the bio-recognition element, the driving forces for the adsorption process and the detection mode of the bio-recognition event. This work is focused on the study of the adsorption mechanism of the His-tag H49 Chagas antigen on Ni(II) modified substrates. In order to construct the bio-functional surface, the gen of the H49 Chagas antigen was modified to incorporate His6 moiety at the N-terminal (His6-H49). Then, its physical adsorption and bio-affinity interaction with the solid substrate was studied by reflectometry. Besides His-Ni(II) bio-affinity interactions, His6-H49 was also physically adsorbed on Ni(II) modified substrates, leading to randomly oriented antigens. These loosely attached bio-molecules were partially removed using conditions of electrostatic repulsion. On the other hand, bio-affinity interactions, resulting in site-oriented molecules on the substrate, were only removable by specific competitors for Ni(II) surface sites. Finally, the surface bio-activity was determined from the peak separations of voltammetry waves due to the change of the electron transfer kinetics of a redox probe through the bio-functional surface (working electrode). Fil: Valenti, Laura Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina Fil: Smania, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina

How to cite

Elegí el formato que necesitás y copiá la referencia al portapapeles.

APA 7

Valenti, L. E. E. A. (2013). Driving forces for the adsorption of a His-tag Chagas antigen: a rational approach to design bio-functional surfaces. http://hdl.handle.net/11336/25843

MLA

Valenti, Laura Elisa et al. "Driving forces for the adsorption of a His-tag Chagas antigen: a rational approach to design bio-functional surfaces." 2013. http://hdl.handle.net/11336/25843.

Chicago

Valenti, Laura Elisa et al. 2013. "Driving forces for the adsorption of a His-tag Chagas antigen: a rational approach to design bio-functional surfaces.". http://hdl.handle.net/11336/25843.

Harvard

Valenti, L. E. E. A. 2013, Driving forces for the adsorption of a His-tag Chagas antigen: a rational approach to design bio-functional surfaces, Elsevier Science, available at: http://hdl.handle.net/11336/25843 [Accessed 7 Aug. 2026].

Share and print

Save the record, copy its permanent link, or print it as a PDF.

Export reference

You can export the record in common formats for use in a reference manager.

RIS BibTeX

Resource details

Bibliographic information to help confirm that this is the correct material.

Title
Driving forces for the adsorption of a His-tag Chagas antigen: a rational approach to design bio-functional surfaces
Author / contributors
Valenti, Laura Elisa et al
Publisher
Elsevier Science
Publication year
2013
ISSN
0927-7765
ISSN
0927-7765
Language
English

Subjects

Explore related resources through these subjects.

H49 Antigen; Ni(Ii) Modified Substrates; Adsorption Mechanism; Bio-Affinity Interaction; Immunosensor; Direct Non-Labeled Detection; https://purl.org/becyt/ford/1.4; https://purl.org/becyt/ford/1
Copied