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Set of Classical PCRs for Detection of Mutations in Candida glabrata FKS Genes Linked with Echinocandin Resistance

Dudiuk, Catiana Beatriz et al · American Society for Microbiology · 2014

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Author / responsible party
Dudiuk, Catiana Beatriz et al
Publisher
American Society for Microbiology
Year
2014
ISSN
2609-2614
ISSN
2609-2614
Language
English

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Clinical echinocandin resistance among Candida glabrata strains is increasing, especially in the United States. Antifungal susceptibility testing is considered mandatory to guide therapeutic decisions. However, these methodologies are not routinely performed in the hospital setting due to their complexity and the time needed to obtain reliable results. Echinocandin failure in C. glabrata is linked exclusively to Fks1p and Fks2p amino acid substitutions, and detection of such substitutions would serve as a surrogate marker to identify resistant isolates. In this work, we report an inexpensive, simple, and quick classical PCR set able to objectively detect the most common mechanisms of echinocandin resistance in C. glabrata within 4 h. The usefulness of this assay was assessed using a blind collection of 50 C. glabrata strains, including 16 FKS1 and/or FKS2 mutants. Fil: Dudiuk, Catiana Beatriz. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Gamarra, Maria Soledad. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina

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APA 7

Dudiuk, C. B. E. A. (2014). Set of Classical PCRs for Detection of Mutations in Candida glabrata FKS Genes Linked with Echinocandin Resistance. http://hdl.handle.net/11336/31060

MLA

Dudiuk, Catiana Beatriz et al. "Set of Classical PCRs for Detection of Mutations in Candida glabrata FKS Genes Linked with Echinocandin Resistance." 2014. http://hdl.handle.net/11336/31060.

Chicago

Dudiuk, Catiana Beatriz et al. 2014. "Set of Classical PCRs for Detection of Mutations in Candida glabrata FKS Genes Linked with Echinocandin Resistance.". http://hdl.handle.net/11336/31060.

Harvard

Dudiuk, C. B. E. A. 2014, Set of Classical PCRs for Detection of Mutations in Candida glabrata FKS Genes Linked with Echinocandin Resistance, American Society for Microbiology, available at: http://hdl.handle.net/11336/31060 [Accessed 6 Aug. 2026].

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Title
Set of Classical PCRs for Detection of Mutations in Candida glabrata FKS Genes Linked with Echinocandin Resistance
Author / contributors
Dudiuk, Catiana Beatriz et al
Publisher
American Society for Microbiology
Publication year
2014
ISSN
2609-2614
ISSN
2609-2614
Language
English

Subjects

Explore related resources through these subjects.

Candida; Glabrata; Resistencia; Equinocandinas; https://purl.org/becyt/ford/1.6; https://purl.org/becyt/ford/1
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