Effect of foliar application of phosphites in "hayward" kiwifruit in storage and shelf life: analysis of pectin composition

The kiwifruit (Actinidia chinensis var chinensis cv. Hayward), a climacteric fruit, can be harvested at physiological maturity and maintain its quality for up to six months at cold storage. The length of the storage period depends, among others, on cell wall composition and structure, which impacts on texture and softening. Indeed, solubilization and degradation of pectins occur during fruit softening, leading to disintegration of the cell wall. Pectin is a complex heterogeneous polymer that can have different interactions within the cell wall as free pectin, bound to starch, attached by calcium bridges and bound to cellulose via hydrogen bonds. The phosphites activate the synthesis of compounds that reinforce cell walls, like pectin and lignin. The aim of this work was to study the effect of foliar application of phosphite on pectin composition in cold storage and shelf life. Plants were foliar sprayed (six weekly applications) 100 days after blooming, with 0.3% potassium phosphite (KPhi; 30% P2O5, 20% K2O) or water (Control). Fruits were harvested at physiological maturity and stored for 5 and 6 months (5M and 6M) at 0°C and 90-95% RH. Kiwifruit was analyzed at the end of each storage period (ES) and its shelf life (SL, 7 day at 20°C). Samples of outer pericarp tissue were frozen and ground using liquid N2. A chemical solvent method was used to successively extract cell walls and determine the composition of pectin. The cell wall material (CWM) was obtained by the enzymes inactivation with a mixture of phenol:acetic acid:water (PAW) and water soluble pectin fraction (W-SP) was recovered. To remove the kiwifruit starch and extract their bound pectin (S-SP), a solution of dimethyl sulfoxide was used. The Na2CO3 was added to obtain the pectin attached by tightly bonds and calcium bridges (C-SP). Each extract was dialysed 5 days, lyophilized and weighed. The results showed that KPhi treatment increased the yields of total pectins respect to the Control and also resulted lower at 5M respect to 6M of storage. At shelf life, pectin yield decreased in all treatments compared to end of storage. The yield of W-SP fractions was lower in SL than in ES, and resulted highest in KPhi treatment at 6M. The yield S-SP fraction was lowest in KPhi and in SL. This could be due to the enzymatic degradation of starch. The proportion of C-SP yield also was lower in KPhi but was higher in SL. The yield of CWM decreased from the 5M to 6M and increase at SF with the application of KPhi. These results suggest that KPhi treatment promote the pectin biosynthesis and their release in shelf life. After 5-6 months of cold storage, Hayward kiwifruit enters in its last ripening/over-ripening stage related to senescence, led by cell wall disintegration. In conclusion, KPhi treatment is suggested to be used in order to maintain the firmmess Hayward kiwifruit, at least until 5 months in cold storage prior shelf life and its consumption. Fil: Rivas Velasquez, Manuel de Jesus. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina Fil: Paz, Cosme Daniel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina