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Measurement of the glycogen synthetic pathway in permeabilized cells of cyanobacteria

Gomez Casati, Diego Fabian et al · Wiley Blackwell Publishing, Inc · 2001

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Author / responsible party
Gomez Casati, Diego Fabian et al
Publisher
Wiley Blackwell Publishing, Inc
Year
2001
ISSN
0378-1097
ISSN
0378-1097
Language
English

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AbstractA simple, rapid and reliable procedure for permeabilizing cyanobacterial cells and measuring the glycogen synthetic pathway in situ, is presented. Cells from Anabaena sp. strain PCC 7120 were permeabilized with a mixture of toluene:ethanol (1:4 v/v). Fluorescence microscopy of cells incubated with fluorescein diacetate showed Anabaena non-permeabilized cells as green fluorescents, whereas permeabilized (viable) cells exhibited the intrinsic red fluorescence. Labelled K-1,4-glucan was recovered when permeabilized cells were incubated with the substrates of ADP-glucose pyrophosphorylase or glycogen synthase. The kinetic and regulatory properties of both enzymes could be reproduced in situ. The simplicity of the procedure and the ability to measure in situ glucan fluxes show the methodology as useful for studying the intracellular regulation of storage polysaccharides in a photosynthetic prokaryote. ß 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.Keywords: Glycogen; Starch synthesis; Cyanobacteria; Anabaena PCC 7120; Glycogen synthase; Permeabilized cell ; Metabolic £ux measurement;ADP-glucose pyrophosphorylase Fil: Gomez Casati, Diego Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: Aon, Miguel A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina

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APA 7

Gomez Casati, D. F. E. A. (2001). Measurement of the glycogen synthetic pathway in permeabilized cells of cyanobacteria. http://hdl.handle.net/11336/85680

MLA

Gomez Casati, Diego Fabian et al. "Measurement of the glycogen synthetic pathway in permeabilized cells of cyanobacteria." 2001. http://hdl.handle.net/11336/85680.

Chicago

Gomez Casati, Diego Fabian et al. 2001. "Measurement of the glycogen synthetic pathway in permeabilized cells of cyanobacteria.". http://hdl.handle.net/11336/85680.

Harvard

Gomez Casati, D. F. E. A. 2001, Measurement of the glycogen synthetic pathway in permeabilized cells of cyanobacteria, Wiley Blackwell Publishing, Inc, available at: http://hdl.handle.net/11336/85680 [Accessed 10 Aug. 2026].

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Title
Measurement of the glycogen synthetic pathway in permeabilized cells of cyanobacteria
Author / contributors
Gomez Casati, Diego Fabian et al
Publisher
Wiley Blackwell Publishing, Inc
Publication year
2001
ISSN
0378-1097
ISSN
0378-1097
Language
English

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