Micronuclei and sister chromatid exchange in lymphocytes from physicians exposed to ionizing radiation

Abstract Objective Occupational exposure to ionizing radiation is a known risk factor for genomic instability. This study evaluated whether physicians occupationally exposed to ionizing radiation exhibit higher frequencies of chromosomal alterations compared to unexposed hospital workers, utilizing micronuclei and sister chromatid exchanges as biomarkers. Methods Peripheral blood samples were analyzed from 50 participants: 25 physicians exposed to ionizing radiation (radiologists) and a control group of 25 sex- and age-matched hospital staff with no history of occupational exposure. The cytokinesis-block micronucleus and sister chromatid exchange assays were employed to assess genomic instability. Results No significant differences were observed in hematological parameters between the two groups. However, the frequencies of both micronuclei and sister chromatid exchanges were significantly higher in the exposed physicians compared to the unexposed control group. Chronic occupational exposure to ionizing radiation is associated with measurable signs of genomic instability. These findings highlight the necessity of implementing regular biomonitoring programs for exposed medical personnel to detect early genetic alterations and mitigate potential health risks.