News Help Institutional access
Back to results
Bibliographic record · Consultation and access
Artículo

Bioremediation approaches in a laboratory activity for the Industrial Biotechnology and Applied Microbiology (IBAM) course

Raiger Iustman, Laura Judith et al · American Society for Microbiology · 2013

Supplementary material available
Quick overview. Review the resource’s basic details, then access the content using the main button. This page shows only the information needed to identify, cite, and open the work.
Author / responsible party
Raiger Iustman, Laura Judith et al
Publisher
American Society for Microbiology
Year
2013
ISSN
1935-7877
ISSN
1935-7877
Language
English

Resource access

Elegí el proveedor desde el que querés acceder.

CONICET Digital CONICET Digital OAI-PMH
Entrar por CONICET Digital
DOAJ DOAJ - Open Access Journals
Entrar por DOAJ
Main access

Supplementary material available

El enlace apunta a material asociado, anexos, tablas, datos o página complementaria. No se marca como libro/texto completo.
Open material
Otras opciones de acceso Elegí el proveedor disponible para esta ficha.
Importación CSV DOAJ - Open Access Journals
Acceder por Importación CSV
DOAJ CSV Export DOAJ - Open Access Journals
Acceder por DOAJ CSV Export

Other available options

When the resource is available on more than one platform, you can choose where to open it.

Importación CSV DOAJ - Open Access Journals Access available
Open
DOAJ CSV Export DOAJ - Open Access Journals Access available
Open

Summary

Descripción general del contenido del recurso.

Industrial Biotechnology and Applied Microbiology is an optional 128h-course for Chemistry and Biology students at the Faculty of Sciences, University of Buenos Aires, Argentina. This course is usually attended by 25 students, working in teams of two. The curriculum, with 8 lab exercises, includes an oil bioremediation practice covering an insight of bioremediation processes: the influence of pollutants on autochthonous microbiota, biodegrader isolation and biosurfactant production for bioavailability understanding. The experimental steps are: (A) evaluation of microbial tolerance to pollutants by constructing pristine soil microcosms contaminated with diesel or xylene and (B) isolation of degraders and biosurfactant production analysis. To check microbial tolerance, microcosms are incubated during one week at 25-28ºC. Samples are collected at 0, 4 and every 48 h for CFU/g soil testing. An initial decrease of total CFU/g related to toxicity is noticed. At the end of the experiment, a recovery of the CFU number is observed, evidencing enrichment in biodegraders. Some colonies from the CFU counting plates are streaked in M9-agar with diesel as sole carbon source. After a week, isolates are inoculated on M9-Broth supplemented with diesel to induce biosurfactant production. Surface tension and Emulsification Index are measured in culture supernatants to visualize tensioactive effect of bacterial products. Besides the improvement in the good microbiological practices, the students show enthusiasm in different aspects, depending on their own interests. While biology students explore and learn new concepts on solubility, emulsions and bioavailability, chemistry students show curiosity in bacterial behavior and manipulation of microorganisms for environmental benefits. Fil: Raiger Iustman, Laura Judith. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: López, Nancy Irene. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

How to cite

Elegí el formato que necesitás y copiá la referencia al portapapeles.

APA 7

Raiger Iustman, L. J. E. A. (2013). Bioremediation approaches in a laboratory activity for the Industrial Biotechnology and Applied Microbiology (IBAM) course. http://hdl.handle.net/11336/21141

MLA

Raiger Iustman, Laura Judith et al. "Bioremediation approaches in a laboratory activity for the Industrial Biotechnology and Applied Microbiology (IBAM) course." 2013. http://hdl.handle.net/11336/21141.

Chicago

Raiger Iustman, Laura Judith et al. 2013. "Bioremediation approaches in a laboratory activity for the Industrial Biotechnology and Applied Microbiology (IBAM) course.". http://hdl.handle.net/11336/21141.

Harvard

Raiger Iustman, L. J. E. A. 2013, Bioremediation approaches in a laboratory activity for the Industrial Biotechnology and Applied Microbiology (IBAM) course, American Society for Microbiology, available at: http://hdl.handle.net/11336/21141 [Accessed 6 Aug. 2026].

Share and print

Save the record, copy its permanent link, or print it as a PDF.

Export reference

You can export the record in common formats for use in a reference manager.

RIS BibTeX

Resource details

Bibliographic information to help confirm that this is the correct material.

Title
Bioremediation approaches in a laboratory activity for the Industrial Biotechnology and Applied Microbiology (IBAM) course
Author / contributors
Raiger Iustman, Laura Judith et al
Publisher
American Society for Microbiology
Publication year
2013
ISSN
1935-7877
ISSN
1935-7877
Language
English

Subjects

Explore related resources through these subjects.

OIL BIOREMEDIATION; HYDROCARBON BIODEGRADATION; BIOSURFACTANT; SURFACE TENSION; https://purl.org/becyt/ford/2.8; https://purl.org/becyt/ford/2
Copied