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DNA polymorphisms amplified by arbitrary primers are useful as genetic markers

John G. Williams; Anne R. Kubelik; Kenneth J. Livak; J. Antoni Rafalski; Scott Tingey · Nucleic Acids Research · 1990

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Author / responsible party
John G. Williams; Anne R. Kubelik; Kenneth J. Livak; J. Antoni Rafalski; Scott Tingey
Publisher
Nucleic Acids Research
Year
1990
Language
English

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Molecular genetic maps are commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs) among the progeny of a sexual cross. Here we describe a new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. We suggest that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.

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APA 7

Williams, J. G, Kubelik, A. R, Livak, K. J, Rafalski, J. A, & Tingey, S. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. https://doi.org/10.1093/nar/18.22.6531

MLA

Williams, John G, et al. "DNA polymorphisms amplified by arbitrary primers are useful as genetic markers." 1990. https://doi.org/10.1093/nar/18.22.6531.

Chicago

Williams, John G, Anne R. Kubelik, Kenneth J. Livak, J. Antoni Rafalski, and Scott Tingey. 1990. "DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.". https://doi.org/10.1093/nar/18.22.6531.

Harvard

Williams, J. G. et al. 1990, DNA polymorphisms amplified by arbitrary primers are useful as genetic markers, Nucleic Acids Research, available at: https://doi.org/10.1093/nar/18.22.6531 [Accessed 8 Aug. 2026].

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Title
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
Author / contributors
John G. Williams; Anne R. Kubelik; Kenneth J. Livak; J. Antoni Rafalski; Scott Tingey
Publisher
Nucleic Acids Research
Publication year
1990
Language
English

Subjects

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Biology; RAPD; Genetics; Restriction fragment length polymorphism; DNA; Genetic marker; Polymorphism (computer science); Amplified fragment length polymorphism; Polymerase chain reaction; DNA sequencing; Molecular biology; Gene
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