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Artículo

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei

John David Dignam; Russell M. Lebovitz; R G Roeder · Nucleic Acids Research · 1983

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Author / responsible party
John David Dignam; Russell M. Lebovitz; R G Roeder
Publisher
Nucleic Acids Research
Year
1983
Language
English

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We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2. The extract also directs accurate transcription initiation from other adenovirus promoters and cellular promoters. The extract also directs accurate transcription initiation from class III promoters (tRNA and Ad 2 VA).

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APA 7

Dignam, J. D, Lebovitz, R. M, & Roeder, R. G. (1983). Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. https://doi.org/10.1093/nar/11.5.1475

MLA

Dignam, John David, et al. "Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei." 1983. https://doi.org/10.1093/nar/11.5.1475.

Chicago

Dignam, John David, Russell M. Lebovitz, and R G Roeder. 1983. "Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.". https://doi.org/10.1093/nar/11.5.1475.

Harvard

Dignam, J. D, Lebovitz, R. M. and Roeder, R. G. 1983, Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei, Nucleic Acids Research, available at: https://doi.org/10.1093/nar/11.5.1475 [Accessed 9 Aug. 2026].

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Resource details

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Title
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei
Author / contributors
John David Dignam; Russell M. Lebovitz; R G Roeder
Publisher
Nucleic Acids Research
Publication year
1983
Language
English

Subjects

Explore related resources through these subjects.

Promoter; Biology; Transcription (linguistics); RNA polymerase II; Molecular biology; Transcription factor II D; RNA polymerase; General transcription factor; RNA; Genetics; Gene; Gene expression
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