News Help Institutional access
Back to results
Bibliographic record · Consultation and access
Artículo

Development and validation of an analytical method for the simultaneous determination of benzoylecgonine and ecgonine methyl ester in human urine by gas chromatography/mass spectrometry

Montes de Oca Porto, Rodny et al · SEDICI UNLP · 2010

Supplementary material available
Quick overview. Review the resource’s basic details, then access the content using the main button. This page shows only the information needed to identify, cite, and open the work.
Author / responsible party
Montes de Oca Porto, Rodny et al
Publisher
SEDICI UNLP
Year
2010
Language
English

Resource access

Open the content from the main option or choose another available source.

SEDICI UNLP SEDICI UNLP OAI-PMH
Entrar por SEDICI UNLP
Main access

Supplementary material available

El enlace apunta a material asociado, anexos, tablas, datos o página complementaria. No se marca como libro/texto completo.
Open material

Summary

Descripción general del contenido del recurso.

A simple and rapid procedure has been developed and validated for the simultaneous determination of benzoylecgonine and ecgonine methyl ester in human urine samples. After extraction and sample cleanup by solid-phase extraction (SPE), the extracts were derivatized with 50 uL of MTBSTFA and analyzed by gas chromatography/mass spectrometry (GC/MS). The limit of detection (LOD) was 50 ng/mL for both metabolites. The recoveries were 81% and 75.3% for benzoylecgonine and ecgonine methyl ester, respectively. The intra-assay precision (% relative standard deviation) at two different added amounts (200 and 1000 ng/mL) in urine matrix varied from 4.8 to 8.4 and from 4.4 to 5.7, respectively. The mass spectra obtained for each compound have many diagnostics ions with relative abundance in accordance with the WADA requirements. Colegio de Farmacéuticos de la Provincia de Buenos Aires

How to cite

Elegí el formato que necesitás y copiá la referencia al portapapeles.

APA 7

Montes de Oca Porto, R. E. A. (2010). Development and validation of an analytical method for the simultaneous determination of benzoylecgonine and ecgonine methyl ester in human urine by gas chromatography/mass spectrometry. http://sedici.unlp.edu.ar/handle/10915/7984

MLA

Montes de Oca Porto, Rodny et al. "Development and validation of an analytical method for the simultaneous determination of benzoylecgonine and ecgonine methyl ester in human urine by gas chromatography/mass spectrometry." 2010. http://sedici.unlp.edu.ar/handle/10915/7984.

Chicago

Montes de Oca Porto, Rodny et al. 2010. "Development and validation of an analytical method for the simultaneous determination of benzoylecgonine and ecgonine methyl ester in human urine by gas chromatography/mass spectrometry.". http://sedici.unlp.edu.ar/handle/10915/7984.

Harvard

Montes de Oca Porto, R. E. A. 2010, Development and validation of an analytical method for the simultaneous determination of benzoylecgonine and ecgonine methyl ester in human urine by gas chromatography/mass spectrometry, SEDICI UNLP, available at: http://sedici.unlp.edu.ar/handle/10915/7984 [Accessed 8 Aug. 2026].

Share and print

Save the record, copy its permanent link, or print it as a PDF.

Export reference

You can export the record in common formats for use in a reference manager.

RIS BibTeX

Resource details

Bibliographic information to help confirm that this is the correct material.

Title
Development and validation of an analytical method for the simultaneous determination of benzoylecgonine and ecgonine methyl ester in human urine by gas chromatography/mass spectrometry
Author / contributors
Montes de Oca Porto, Rodny et al
Publisher
SEDICI UNLP
Publication year
2010
Language
English

Subjects

Explore related resources through these subjects.

Farmacia; benzoylecgonine; ecgonine methyl ester; doping control; gas chromatography/mass spectrometry; Medicamentos; Doping en los deportes; Farmacología; Bioquímica
Copied