News Help Institutional access
Back to results
Bibliographic record · Consultation and access
Artículo

Standardization of Caco-2 cell culture as in vitro model for intestinal permeability

Rodríguez, Yanina I. et al · SEDICI UNLP · 2011

Open-access full text
Quick overview. Review the resource’s basic details, then access the content using the main button. This page shows only the information needed to identify, cite, and open the work.
Author / responsible party
Rodríguez, Yanina I. et al
Publisher
SEDICI UNLP
Year
2011
Language
English

Resource access

Open the content from the main option or choose another available source.

SEDICI UNLP SEDICI UNLP OAI-PMH
Entrar por SEDICI UNLP
Main access

Open-access full text

Texto completo identificado como acceso abierto.
Open text

Summary

Descripción general del contenido del recurso.

The aim of this study was to find out the optimal experimental conditions for Caco-2 cell culture (time and density) and permeability assays (diffusion system and drug concentration) in order to study the in vitro drugs permeability as a predictive method for drug absorption across intestinal epithelium. The integrity of the monolayers used in each assay was determined by measuring the transepithelial electrical resistance (TEER) and the permeability of the atenolol-a drug which is transported across the monolayers by the paracellular pathway-. The best working condition was obtained with a cell seeding of 7.10<sup>4</sup> cells/insert in a vertical difussion chamber. In such context, the monolayers had a TEER higher than 550 Ω.cm<sup>2</sup> and the apparent permeability coefficient of atenolol was 0.71 ± 0.19 x 10<sup>-6</sup> cm/seg. Colegio de Farmacéuticos de la Provincia de Buenos Aires

How to cite

Elegí el formato que necesitás y copiá la referencia al portapapeles.

APA 7

Rodríguez, Y. I. E. A. (2011). Standardization of Caco-2 cell culture as in vitro model for intestinal permeability. http://sedici.unlp.edu.ar/handle/10915/8097

MLA

Rodríguez, Yanina I. et al. "Standardization of Caco-2 cell culture as in vitro model for intestinal permeability." 2011. http://sedici.unlp.edu.ar/handle/10915/8097.

Chicago

Rodríguez, Yanina I. et al. 2011. "Standardization of Caco-2 cell culture as in vitro model for intestinal permeability.". http://sedici.unlp.edu.ar/handle/10915/8097.

Harvard

Rodríguez, Y. I. E. A. 2011, Standardization of Caco-2 cell culture as in vitro model for intestinal permeability, SEDICI UNLP, available at: http://sedici.unlp.edu.ar/handle/10915/8097 [Accessed 8 Aug. 2026].

Share and print

Save the record, copy its permanent link, or print it as a PDF.

Export reference

You can export the record in common formats for use in a reference manager.

RIS BibTeX

Resource details

Bibliographic information to help confirm that this is the correct material.

Title
Standardization of Caco-2 cell culture as in vitro model for intestinal permeability
Author / contributors
Rodríguez, Yanina I. et al
Publisher
SEDICI UNLP
Publication year
2011
Language
English

Subjects

Explore related resources through these subjects.

Farmacia; Técnicas de Cultivo de Célula; atenolol; biopharmaceutics classification system; Caco-2; permeability; Permeabilidad
Copied