Determination of galangin in commercial extracts of Alpinia officinarum by RP-HPLC-DAD

Alpinia officinarum has been used by long time in the traditional medicine and several commercial extracts containing it are available on the market. In this paper a reversed phase high performance liquid chromatography-diode array detection (RP-HPLC-DAD) method for Alpinia officinarum preparations was proposed using galangin as a marker. The quantification was undertaken by a combination of RP-HPLC-DAD. The LC system employed a Gemini Phenomenex RP-18 column with phosphoric acid, tetrahydrofuran and acetonitrile as mobile phase and detection at 267 nm. The method validation was throughout satisfactory. The determination coefficient was r² = 0.998 with LOD 0.48 µg mL^-1 and LOQ 1.59 µg mL^-1 The results of precision (RSD < 2.82) and accuracy (99.9 %, 100.4 %, 102.2 %) with . RSD less than 2.7 %, were satisfactory. The analytical method was applied to commercial extracts, and was suitable to provide qualitative and quantitative information for the quality control to commercial preparations. Colegio de Farmacéuticos de la Provincia de Buenos Aires