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Qualitative and quantitative analyses of three bioactive compounds in traditional chinese medicine gamboge by HPLC-PDA-ESI/MS<sup>n</sup>

Zhou, An et al · SEDICI UNLP · 2011

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Author / responsible party
Zhou, An et al
Publisher
SEDICI UNLP
Year
2011
Language
English

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A high performance liquid chromatography photo diode array UV detection electrospray ionization tandem mass spectrometry (HPLC-PDA-ESI/MS<sup>n</sup>) method was developed and validated for the quality evaluation of gamboge (dryed resin exuded from the stems of Garcinia hanburyi). The contents of the three bioactive constituents (gambogenic acid, R-gambogic acid and S-gambogic) were determined by using HPLC–PDA, and their chemical structures were identified by HPLC-PDA-ESI/MS<sup>n</sup>.The limits of detection and quantitation were between 0.039-0.048 μg/mL and 0.13-0.16 μg/mL. The intra- and inter- assay precisions, in terms of percent relative standard deviation, are less than 3.7 and 4.8 %, respectively . The accuracy, in terms of recovery percentage, ranged from 96.86 to 101.70 %. Good linearity (correlation coefficient > 0.9996) for each calibration curve of standards. HPLC-PDA-ESI/MS<sup>n</sup> was use to analyze caged xanthones in gamboge. A total of 16 peaks were identified or tentatively characterized. The results indicated that the method could be considered to be a simple, rapid and reliable method for the quality evaluation of gamboge. Colegio de Farmacéuticos de la Provincia de Buenos Aires

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APA 7

Zhou, A. E. A. (2011). Qualitative and quantitative analyses of three bioactive compounds in traditional chinese medicine gamboge by HPLC-PDA-ESI/MSn. http://sedici.unlp.edu.ar/handle/10915/8336

MLA

Zhou, An et al. "Qualitative and quantitative analyses of three bioactive compounds in traditional chinese medicine gamboge by HPLC-PDA-ESI/MSn." 2011. http://sedici.unlp.edu.ar/handle/10915/8336.

Chicago

Zhou, An et al. 2011. "Qualitative and quantitative analyses of three bioactive compounds in traditional chinese medicine gamboge by HPLC-PDA-ESI/MSn.". http://sedici.unlp.edu.ar/handle/10915/8336.

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Zhou, A. E. A. 2011, Qualitative and quantitative analyses of three bioactive compounds in traditional chinese medicine gamboge by HPLC-PDA-ESI/MSn, SEDICI UNLP, available at: http://sedici.unlp.edu.ar/handle/10915/8336 [Accessed 7 Aug. 2026].

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Title
Qualitative and quantitative analyses of three bioactive compounds in traditional chinese medicine gamboge by HPLC-PDA-ESI/MS<sup>n</sup>
Author / contributors
Zhou, An et al
Publisher
SEDICI UNLP
Publication year
2011
Language
English

Subjects

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Farmacia; gamboge; garcinia hanburyi; HPLC?PDA?ESI/MSn; quality control; traditional chinese medicine; Plantas Medicinales; Cromatografía; Estudios de Evaluación
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