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Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray

Mark Schena; Dari Shalon; Ronald W. Davis; Patrick O. Brown · Science · 1995

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Author / responsible party
Mark Schena; Dari Shalon; Ronald W. Davis; Patrick O. Brown
Publisher
Science
Year
1995
Language
English

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A high-capacity system was developed to monitor the expression of many genes in parallel. Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. Because of the small format and high density of the arrays, hybridization volumes of 2 microliters could be used that enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA. Differential expression measurements of 45 Arabidopsis genes were made by means of simultaneous, two-color fluorescence hybridization.

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APA 7

Schena, M, Shalon, D, Davis, R. W, & Brown, P. O. (1995). Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray. https://doi.org/10.1126/science.270.5235.467

MLA

Schena, Mark, et al. "Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray." 1995. https://doi.org/10.1126/science.270.5235.467.

Chicago

Schena, Mark, Dari Shalon, Ronald W. Davis, and Patrick O. Brown. 1995. "Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray.". https://doi.org/10.1126/science.270.5235.467.

Harvard

Schena, M. et al. 1995, Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray, Science, available at: https://doi.org/10.1126/science.270.5235.467 [Accessed 7 Aug. 2026].

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Title
Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray
Author / contributors
Mark Schena; Dari Shalon; Ronald W. Davis; Patrick O. Brown
Publisher
Science
Publication year
1995
Language
English

Subjects

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DNA microarray; Gene; Gene expression; Microarray; DNA; Biology; Messenger RNA; Molecular biology; Arabidopsis; Nucleic acid thermodynamics; RNA; Computational biology
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