Imaging Intracellular Fluorescent Proteins at Nanometer Resolution

Eric Betzig; George H. Patterson; Rachid Sougrat; O. Wolf Lindwasser; Scott G. Olenych; Juan S. Bonifacino; Michael W. Davidson; Jennifer Lippincott‐Schwartz · Science · 2006

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Author / responsible party

Eric Betzig; George H. Patterson; Rachid Sougrat; O. Wolf Lindwasser; Scott G. Olenych; Juan S. Bonifacino; Michael W. Davidson; Jennifer Lippincott‐Schwartz

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Science

Year

2006

Language

English

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We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to approximately 2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was then assembled into a superresolution image. We used this method--termed photoactivated localization microscopy--to image specific target proteins in thin sections of lysosomes and mitochondria; in fixed whole cells, we imaged vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane.

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APA 7

Betzig, E, Patterson, G. H, Sougrat, R, Lindwasser, O. W, Olenych, S. G, Bonifacino, J. S, Davidson, M. W, & Lippincott‐Schwartz, J. (2006). Imaging Intracellular Fluorescent Proteins at Nanometer Resolution. https://doi.org/10.1126/science.1127344

MLA

Betzig, Eric, et al. "Imaging Intracellular Fluorescent Proteins at Nanometer Resolution." 2006. https://doi.org/10.1126/science.1127344.

Chicago

Betzig, Eric, George H. Patterson, Rachid Sougrat, O. Wolf Lindwasser, Scott G. Olenych, Juan S. Bonifacino, Michael W. Davidson, and Jennifer Lippincott‐Schwartz. 2006. "Imaging Intracellular Fluorescent Proteins at Nanometer Resolution.". https://doi.org/10.1126/science.1127344.

Harvard

Betzig, E. et al. 2006, Imaging Intracellular Fluorescent Proteins at Nanometer Resolution, Science, available at: https://doi.org/10.1126/science.1127344 [Accessed 7 Aug. 2026].

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Title: Imaging Intracellular Fluorescent Proteins at Nanometer Resolution

Author / contributors: Eric Betzig; George H. Patterson; Rachid Sougrat; O. Wolf Lindwasser; Scott G. Olenych; Juan S. Bonifacino; Michael W. Davidson; Jennifer Lippincott‐Schwartz

Publisher: Science

Publication year: 2006

Language: English

Subjects

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Vinculin; Lamellipodium; Microscopy; Actin; Superresolution; Resolution (logic); Super-resolution microscopy; Photoactivated localization microscopy; Intracellular; Biophysics; Cell biology; Chemistry