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Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction

R. Carvalho et al · Universidade Federal de Minas Gerais, Escola de Veterinária · 2000

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Author / responsible party
R. Carvalho et al
Publisher
Universidade Federal de Minas Gerais, Escola de Veterinária
Year
2000
ISSN
1678-4162
ISSN
1678-4162
Language
English
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The genome of one equine embryo and three equine semen specimens collected from a Brazilian farm were tested by polymerase chain reaction (PCR) for the presence of EHV-1 and EHV-4-specific timidine kinase (TK) sequences. The PCR detected specific EHV-1 TK gene sequences in all samples tested. The peripheral blood leukocytes (PBL) of the embryo donor mare also was amplified by EHV-1 TK primers. Infectious virus was not recovered from any specimens. The animals did not show any clinical signal of EHV-1 or EHV-4 infections. EHV-4 was not detected in the studied specimens. The results indicate that PCR was more sensitive than virus isolation in cell culture for detecting EHV-1 in semen of carrier horses.

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APA 7

al, R. C. E. (2000). Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction. https://doi.org/10.1590/S0102-09352000000400002

MLA

al, R. Carvalho et. "Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction." 2000. https://doi.org/10.1590/S0102-09352000000400002.

Chicago

al, R. Carvalho et. 2000. "Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction.". https://doi.org/10.1590/S0102-09352000000400002.

Harvard

al, R. C. E. 2000, Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction, Universidade Federal de Minas Gerais, Escola de Veterinária, available at: https://doi.org/10.1590/S0102-09352000000400002 [Accessed 7 Aug. 2026].

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Title
Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction
Author / contributors
R. Carvalho et al
Publisher
Universidade Federal de Minas Gerais, Escola de Veterinária
Publication year
2000
ISSN
1678-4162
ISSN
1678-4162
Language
English

Subjects

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Equine; semen; embryo; EHV-1; EHV-4; PCR
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